CELLULASE COMPLEX OF A MESOPHILIC STREPTOMYCES STRAIN
CELLULASE COMPLEX OF A MESOPHILIC STREPTOMYCES STRAIN
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DOI：
10.1139/m80-028
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发表时间：
1980-01-01
影响因子：
2.8
通讯作者：
KLUEPFEL, D
中科院分区：
文献类型：
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作者：
ISHAQUE, M;KLUEPFEL, D
从自然环境中分离出多种纤维素分解链霉菌。对其中1株中温链霉菌的纤维素酶产酶情况进行了研究。黄灰蝶属flavogriseus。当以1%微晶纤维素（Avicel）作为底物作为浸没培养物生长时，该菌株产生相当大量的β- 1，4-葡聚糖葡聚糖水解酶（EC 3.2.1.4; CM纤维素酶）在细胞外上清液中的浓度增加，并且表现出良好的总体纤维素分解活性，如使用滤纸（FP纤维素酶）和棉花作为底物所测量的。在30 ℃温育72小时后获得最大酶产率。C. FP纤维素酶活性的最适条件为pH5.6和40 ℃。C.全部是β-在培养物的菌丝部分中发现葡萄糖苷酶和纤维二糖酶活性，并且可以通过超声处理以可溶形式获得。
Various cellulolytic streptomycetes were isolated from natural environments. The cellulase production of 1 of these mesophilic Streptomyces strains, identified as S. flavogriseus was studied. When grown as a submerged culture with 1% microcrystalline cellulose (Avicel) as substrate, the strain produced considerable amounts of .beta.-1,4-glucan glucanohydrolase (EC 3.2.1.4; CM cellulase) in the extracellular supernatant and exhibited good overall cellulolytic activity as measured using filter paper (FP cellulase) and cotton as substrates. The maximum enzyme yields were obtained after 72 h of incubation at 30.degree. C. The optimal conditions for the FP cellulase activity were found at pH 5.6 and 40.degree. C. All .beta.-glucosidase and cellobiase activities were found in the mycelial fraction of the culture and could be obtained in soluble form by sonication.