Transcription of hepatitis B virus by RNA polymerase II.
Transcription of hepatitis B virus by RNA polymerase II.
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RNA 聚合酶 II 转录乙型肝炎病毒。
DOI：
10.1128/mcb.3.10.1766-1773.1983
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发表时间：
1983
影响因子：
5.3
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Rall,LB;Standring,DN;Laub,O;Rutter,WJ
我们采用体外无细胞转录系统定位RNA聚合酶II启动子上的B型肝炎病毒基因组。最强的启动子位于表面抗原（HBsAg）基因之前，该基因由一个长的（500个碱基对）表面前区以及成熟的HBsAg编码序列组成。通过使用截短的模板和S1内切酶图谱定位该转录物的来源。在转染实验中证实了启动子的活性，其中通过猿猴病毒40表达载体将完整的HBsAg基因引入猴肾细胞。第二个RNA聚合酶II启动子之前的HBcAg基因也活跃在无细胞系统。在B型肝炎病毒基因组中存在多个启动子，这表明在肝脏和血清中检测到的病毒特异性蛋白的相对水平可能反映了启动子效率的差异或调节。
We employed an in vitro cell-free transcription system to locate RNA polymerase II promoters on the hepatitis B virus genome. The strongest promoter precedes the surface antigen (HBsAg) gene, which is comprised of a long (500 base pairs) presurface region as well as the mature HBsAg coding sequence. The origin of this transcript was localized by using truncated templates and S1 endonuclease mapping. The activity of the promoter was confirmed in transfection experiments in which the complete HBsAg gene was introduced into monkey kidney cells via a simian virus 40 expression vector. A second RNA polymerase II promoter preceding the HBcAg gene was also active in the cell-free system. The presence of multiple promoters in the hepatitis B virus genome suggests that the relative levels of viral-specific proteins detected in liver and serum may reflect differential or regulated promoter efficiency.