Extremely low-frequency electromagnetic fields enhance the proliferation and differentiation of neural progenitor cells cultured from ischemic brains
Extremely low-frequency electromagnetic fields enhance the proliferation and differentiation of neural progenitor cells cultured from ischemic brains
复制标题
极低频电磁场增强缺血性大脑培养的神经祖细胞的增殖和分化
DOI：
10.1097/wnr.0000000000000450
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发表时间：
2015-10-21
期刊：
影响因子：
1.7
通讯作者：
Fan, Wenying
中科院分区：
文献类型：
--
作者：
Cheng, Yannan;Dai, Yiqin;Fan, Wenying
在哺乳动物的大脑中，神经发生在整个胚胎期和成年期持续存在于侧脑室的脑室下区和海马的颗粒区（齿状回）。这两个区域的新生神经前体细胞（NPC）在脑损伤后的结构和功能可塑性以及神经再生中起着关键作用。研究表明，极低频电磁场（ELF-EMF）可以促进成骨、血管生成和心脏干细胞分化，是一种有效的再生治疗手段。本研究旨在观察极低频电磁场对胚胎和成年缺血脑海马神经前体细胞的影响。我们发现，暴露于ELF-EMF（50 Hz，0.4 mT）显着增强的增殖能力在胚胎和缺血的NPC。累积ELF-EMF暴露7天后，神经元分化也得到增强，而胶质细胞分化没有受到明显影响。在ELF-EMF作用下，缺血性NPCs增殖过程中磷酸化Akt表达增加。然而，Akt通路的阻断消除了ELF-EMF诱导的缺血NPC增殖。这些数据表明，ELF-EMF促进缺血性NPC的神经发生，并表明这种作用可能通过Akt途径发生。http://links.lww.com/WNR/A347
In the mammalian brain, neurogenesis persists throughout the embryonic period and adulthood in the subventricular zone of the lateral ventricle and the granular zone (dentate gyrus) of the hippocampus. Newborn neural progenitor cells (NPCs) in the two regions play a critical role in structural and functional plasticity and neural regeneration after brain injury. Previous studies have reported that extremely low-frequency electromagnetic fields (ELF-EMF) could promote osteogenesis, angiogenesis, and cardiac stem cells’ differentiation, which indicates that ELF-EMF might be an effective tool for regenerative therapy. The present studies were carried out to examine the effects of ELF-EMF on hippocampal NPCs cultured from embryonic and adult ischemic brains. We found that exposure to ELF-EMF (50 Hz, 0.4 mT) significantly enhanced the proliferation capability both in embryonic NPCs and in ischemic NPCs. Neuronal differentiation was also enhanced after 7 days of cumulative ELF-EMF exposure, whereas glial differentiation was not influenced markedly. The expression of phosphorylated Akt increased during the proliferation process when ischemic NPCs were exposed to ELF-EMF. However, blockage of the Akt pathway abolished the ELF-EMF-induced proliferation of ischemic NPCs. These data show that ELF-EMF promotes neurogenesis of ischemic NPCs and suggest that this effect may occur through the Akt pathway.Video abstract, Supplemental Digital Content 1, http://links.lww.com/WNR/A347