CLEARANCE AND FATE OF LEUKEMIA-INHIBITORY FACTOR (LIF) AFTER INJECTION INTO MICE
CLEARANCE AND FATE OF LEUKEMIA-INHIBITORY FACTOR (LIF) AFTER INJECTION INTO MICE
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DOI：
10.1002/jcp.1041480315
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发表时间：
1991-09-01
影响因子：
5.6
通讯作者：
METCALF, D
中科院分区：
文献类型：
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作者：
HILTON, DJ;NICOLA, NA;METCALF, D
白血病抑制因子（LIF）对广泛的细胞类型，包括单核细胞和巨核细胞系列的细胞，胚胎干细胞，肝细胞，脂肪细胞和成骨细胞具有增强作用。天然和重组LIF，静脉注射到成年小鼠，有一个初始的半衰期为6-8分钟和一个更长的第二清除相。I-125-LIF从循环中的清除被肾脏、肝脏、肺和脾脏中的快速蓄积以及甲状腺中的更渐进蓄积所阻碍。放射自显影可显示肾小球簇、实质肝细胞、脾红髓、肺泡肺细胞和甲状腺滤泡细胞以及骨腔中的巨核细胞和成骨细胞、胎盘滋养层细胞和脉络丛细胞的标记。尿液中大量不可沉淀的I-125的出现表明肾脏是LIF从体内清除的主要途径。
Leukemia-inhibitory factor (LIF) elicits effects on a broad range of cell types, including cells of the monocytic and megakaryocytic series, embryonal stem cells, hepatocytes, adipocytes, and osteoblasts. Native and recombinant LIF, injected intravenously into adult mice, had an initial half-life of 6-8 min and a more prolonged second clearance phase. Clearance of I-125-LIF from the circulation was paralleled by a rapid accumulation in the kidneys, liver, lungs, and spleen and a more gradual accumulation in the thyroid gland. Labeling of the renal glomerular tufts, parenchymal hepatocytes, splenic red pulp, alveolar pneumocytes, and thyroid follicular cells as well as of megakaryocytes and osteoblasts in the bone cavities, placental trophoblasts, and cells of the choroid plexus was demonstrable autoradiographically. The appearance of a large amount of nonprecipitable I-125 in the urine suggested that the kidneys were the major route of LIF clearance from the body.