GSTP1 affects chemoresistance against camptothecin in human lung adenocarcinoma cells
GSTP1 affects chemoresistance against camptothecin in human lung adenocarcinoma cells
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DOI：
10.1016/j.canlet.2004.05.018
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发表时间：
2004-12-08
期刊：
影响因子：
9.7
通讯作者：
Matsuse, T
中科院分区：
文献类型：
--
作者：
Ishii, T;Teramoto, S;Matsuse, T
谷胱甘肽 S-转移酶 PI (GSTP1) 被称为通过谷胱甘肽缀合形成的异生酶，也是 Jun N 末端激酶 (JNK) 的抑制剂。我们打算研究 GSTP1 是否影响人肺腺癌细胞对喜树碱的化疗耐药性。喜树碱诱导 GSTP1 表达。 GSTP1 的下调会增加 A549 细胞中喜树碱诱导的坏死，但不会增加 PC-14 和 RERF-LC-KJ 细胞中的坏死。 JNK 抑制剂 SP600125 几乎没有改变 A549 细胞中的这种现象，但 L-丁硫氨酸-亚磺酰亚胺几乎消除了这种现象。这些结果表明，GSTP1 通过谷胱甘肽缀合对人肺腺癌亚型中喜树碱诱导的坏死具有保护作用。 (C) 2004 Elsevier Ireland Ltd. 保留所有权利。
Glutathione S-transferase PI (GSTP1) is known as a xenobiotic enzyme through conjugation of glutathione and also as an inhibitor of Jun N-terminal kinase (JNK). We intended to investigate whether GSTP1 affects chemoresistance against camptothecin in human lung adenocarcinoma cells. Camptothecin induced GSTP1 expression. Downregulation of GSTP1 increased necrosis induced by camptothecin in A549 cells but not in PC-14 and RERF-LC-KJ cells. This phenomenon in A549 cells was hardly changed by JNK inhibitor SP600125 but was almost diminished by L-buthionine-sulfoximine. These results Suggest that GSTP1 has protective effects against camptothecin-induced necrosis in subset of human lung adenocarcinoma through glutathione conjugation. (C) 2004 Elsevier Ireland Ltd. All rights reserved.