VIDEO IMAGE-PROCESSING GREATLY ENHANCES CONTRAST, QUALITY, AND SPEED IN POLARIZATION-BASED MICROSCOPY
VIDEO IMAGE-PROCESSING GREATLY ENHANCES CONTRAST, QUALITY, AND SPEED IN POLARIZATION-BASED MICROSCOPY
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DOI：
10.1083/jcb.89.2.346
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发表时间：
1981-01-01
影响因子：
7.8
通讯作者：
INOUE, S
中科院分区：
文献类型：
--
作者：
INOUE, S
具有对比度和黑电平控制的摄像机可以产生具有前所未有的图像质量、分辨率和记录速度的偏振光和差分干涉对比显微镜图像。视频偏振和微分干涉对比显微镜的理论基础和实际方面进行了讨论，并在细胞生物学中的几个应用举例说明。其中包括：Stentor中皮层结构和纤毛跳动的双折射，单个细菌上旋转鞭毛的双折射，培养中棘皮动物（Arbacia punctulata）骨骼骨针的生长和形态发生，裸鳃蜗牛（Hermissenda crassicornis）平衡器官中的纤毛和电活动，以及激活的（Thyone briareus）精子中的顶体反应。
Video cameras with contrast and black level controls can yield polarized light and differential interference contrast microscope images with unprecedented image quality, resolution and recording speed. The theoretical basis and practical aspects of video polarization and differential interference contrast microscopy are discussed and several applications in cell biology are illustrated. These include: birefringence of cortical structures and beating cilia in Stentor, birefringence of rotating flagella on a single bacterium, growth and morphogenesis of echinoderm (Arbacia punctulata) skeletal spicules in culture, ciliary and electrical activity in a balancing organ of a nudibranch snail (Hermissenda crassicornis), and acrosomal reaction in activated (Thyone briareus) sperm.