Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination
Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination
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过表达 PTrx 的转基因大麦增加了发芽过程中根部的铝抗性
DOI：
10.1631/jzus.b1000048
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发表时间：
2010-11-01
影响因子：
5.1
通讯作者：
Wang, Xiang
中科院分区：
文献类型：
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作者：
Li, Qiao-yun;Niu, Hong-bin;Wang, Xiang
采用过表达蓝堇硫氧还蛋白基因（PTrx）的转基因大麦株系LSY-11-1-1，在2 mmol/L AlCl3滤纸处理下，测定了萌发过程中大麦根系的生长、蛋白质氧化、细胞活力和抗氧化酶活性。结果表明：(1)与非转基因大麦相比，LSY-11-1-1促进了大麦根系生长，但经AlCl3处理后，两者均受到严重抑制；(2) LSY-11-1-1的蛋白质氧化程度和细胞活力丧失程度远低于非转基因大麦，表现为LSY-11-1-1的蛋白质羰基含量和Evans蓝吸收量较低；(3)铝胁迫下，LSY-11-1-1根尖过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）活性普遍高于非转基因大麦根尖，但这些抗氧化酶活性在LSY-11-1-1和非转基因大麦中均有不同程度的升高。这些结果表明，过表达PTrx可以通过提高抗氧化酶活性，有效地保护大麦根系免受氧化损伤，从而抑制萌发过程中AlCl3引起的ROS。这些特性提高了PTrx过表达转基因大麦在酸性土壤中降低铝毒性的可能性。
A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl3 treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl3 during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.