Rapid fabrication of a microfluidic device with integrated optical waveguides for DNA fragment analysis
Rapid fabrication of a microfluidic device with integrated optical waveguides for DNA fragment analysis
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DOI：
10.1039/b708485d
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Backhouse, Christopher J.
中科院分区：
文献类型：
--
作者：
Bliss, Christopher L.;McMullin, James N.;Backhouse, Christopher J.
介绍了一种用于激光诱导荧光DNA片段分析的集成液芯光波导微流控装置的制作和性能。该器件是通过聚（二甲基硅氧烷）（PDMS）的软光刻和波导形成在专用通道，通过添加液体PDMS预聚物的较高的折射率。一旦已经制造了母版，微流体芯片可以在不到3小时内生产，而不需要洁净室，然而这种方法提供了比传统的共焦光学组件具有更高性能的光学系统。通过将光纤插入到芯片边缘处的光纤到波导耦合器中来实现光学耦合，并且液体-光纤界面导致低反射和散射损耗。波导传播损耗测量为1.8 dB cm 21（532 nm）和1.0 dB cm 21（633 nm）。由于光纤接口处的损耗，该芯片显示出7.6 dB的平均总耦合损耗。在BK病毒PCR产物的电泳分离和检测中，波导系统实现了570 +/- 30的平均信噪比，而商业共聚焦台式电泳系统实现了330 +/- 30的平均SNR。据我们所知，这是第一次，波导为基础的系统已被证明有一个SNR可比的市售的共焦为基础的微芯片毛细管电泳系统。
The fabrication and performance of a microfluidic device with integrated liquid-core optical waveguides for laser induced fluorescence DNA fragment analysis is presented. The device was fabricated through poly(dimethylsiloxane) (PDMS) soft lithography and waveguides are formed in dedicated channels through the addition of a liquid PDMS pre-polymer of higher refractive index. Once a master has been fabricated, microfluidic chips can be produced in less than 3 h without the requirement for a cleanroom, yet this method provides an optical system that has higher performance than a conventional confocal optical assembly. Optical coupling was achieved through the insertion of optical fibers into fiber-to-waveguide couplers at the edge of the chip and the liquid-fiber interface results in low reflection and scattering losses. Waveguide propagation losses are measured to be 1.8 dB cm 21 (532 nm) and 1.0 dB cm 21 (633 nm). The chip displays an average total coupling loss of 7.6 dB due to losses at the optical fiber interfaces. In the electrophoretic separation and detection of a BK virus PCR product, the waveguide system achieves an average signal-to-noise ratio of 570 +/- 30 whereas a commercial confocal benchtop electrophoresis system achieves an average SNR of 330 +/- 30. To our knowledge, this is the first time that a waveguide-based system has been demonstrated to have a SNR comparable to a commercially available confocal-based system for microchip capillary electrophoresis.