MULTISTATION MULTIPARAMETER FLOW-CYTOMETRY - A CRITICAL-REVIEW AND RATIONALE
MULTISTATION MULTIPARAMETER FLOW-CYTOMETRY - A CRITICAL-REVIEW AND RATIONALE
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DOI：
10.1002/cyto.990030402
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
SHAPIRO, HM
中科院分区：
文献类型：
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作者：
SHAPIRO, HM
在沿着样品流的不同点处由不同波长的光束激发荧光的能力，以及对由此获得的多参数数据进行计算机分析的能力，现在可在来自商业生产商的流式细胞仪/分选系统中获得。对于大多数有经验的流式细胞仪用户来说，这种多参数分析提供了表征混合群体内细胞亚群的问题的最方便的解决方案。多光束的使用有助于从单个细胞内或单个细胞上的几个探针的荧光信号的分辨率，并拓宽了实验者可用的分析替代方案的范围。这篇评论讨论了仪器的历史，现在可测量的参数和用于测量的探针，以及数据分析的方法。所需的灵敏度和精度进行了讨论，导致的结论是，多站，多参数流式细胞术的许多优点，可以在不太复杂，成本较低的仪器，使用功能较弱的来源和较不复杂的计算机硬件比目前纳入商业设备。
The capacity for fluorescence excitation by beams of different wavelengths at separate points along the sample stream, and the capacity for computer analysis of multiparameter data thus obtained, are now available in flow cytometer/sorter systems from commercial producers. It is now readily apparent to most experienced users of flow cytometers that such multiparameter analysis offers the most convenient solution to the problem of characterizing subpopulations of cells within a mixed population. The use of multiple beams facilitates resolution of fluorescence signals from several probes within or upon a single cell and widens the range of analytical alternatives available to experimenters. This critical review discusses the history of the instrumentation, the parameters now measurable and the probes used for their measurement, and the methods for data analysis. Required sensitivity and precision are discussed, leading to the conclusion that many of the advantages of multistation, multiparameter flow cytometry can be made available in less complex and less costly instruments using less powerful sources and less elaborate computer hardware than are presently incorporated in commercial apparatus.