Protective immune responses against protease-like antigens of the murine malaria parasite Plasmodium vinckei.
Protective immune responses against protease-like antigens of the murine malaria parasite Plasmodium vinckei.
复制标题
针对鼠疟疾寄生虫温克疟原虫的蛋白酶样抗原的保护性免疫反应。
DOI：
10.1016/s0264-410x(98)80119-9
复制
发表时间：
1998
期刊：
影响因子：
5.5
通讯作者：
Rosenthal,PJ
中科院分区：
文献类型：
--
作者：
Gor,DO;Li,AC;Rosenthal,PJ
恶性疟原虫丝氨酸重复抗原（serine repeat antigen，SERA）和丝氨酸重复蛋白同源物（serine repeat protein homologue，SERPH）与半胱氨酸蛋白酶在一个高度保守的蛋白酶结构域中具有序列相似性。我们确定了三个SERA同源物从鼠疟原虫vinckei和评估这些蛋白质的蛋白酶结构域的免疫反应。在连续感染和治愈后对P.vinckei产生保护性免疫的小鼠表现出针对SERA同源蛋白酶结构域的体液和细胞介导的应答。用表达这些抗原之一的蛋白酶结构域的鼠伤寒沙门氏菌免疫的小鼠表现出针对该抗原的细胞反应，并增加了针对P. vinckei致命攻击的存活率。我们的研究结果表明，SERA和SERPH的蛋白酶结构域是值得进一步研究的疟疾疫苗的潜在组成部分。
The Plasmodium falciparum proteins serine repeat antigen (SERA) and serine repeat protein homologue (SERPH) have similarity in sequence with cysteine proteases in a well-conserved protease domain. We identified three SERA homologues from the murine malaria parasite Plasmodium vinckei and evaluated immune responses to the protease domains of these proteins. Mice that developed protective immunity to P. vinckei after serial infection and cure demonstrated humoral and cell-mediated responses against the SERA homologue protease domains. Mice immunized with Salmonella typhimurium expressing the protease domain of one of these antigens demonstrated cellular responses against the antigen and increased survival against lethal challenge with P. vinckei. Our results suggest that the protease domains of SERA and SERPH are worthy of additional study as potential components of a malaria vaccine.