A Novel Variant, NDM-5, of the New Delhi Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli ST648 Isolate Recovered from a Patient in the United Kingdom
A Novel Variant, NDM-5, of the New Delhi Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli ST648 Isolate Recovered from a Patient in the United Kingdom
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DOI：
10.1128/aac.05108-11
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发表时间：
2011-12-01
影响因子：
4.9
通讯作者：
Wareham, David W.
中科院分区：
文献类型：
--
作者：
Hornsey, Michael;Phee, Lynette;Wareham, David W.
在一株多重耐药大肠埃希菌ST 648分离株中鉴定出一种新的新德里金属酶（NDM）碳青霉烯酶变体，该分离株从一名近期在印度住院的英国患者的会阴和咽喉中回收。NDM-5与现有的酶不同，因为在位置88（瓦尔-> Leu）和154（Met -> Leu）处发生了取代，并且降低了E. coli TOP10转化子在其天然启动子下表达时对广谱头孢菌素类和碳青霉烯类抗生素的抗性。
A new variant of the New Delhi metallo-enzyme (NDM) carbapenemase was identified in a multidrug-resistant Escherichia coli ST648 isolate recovered from the perineum and throat of a patient in the United Kingdom with a recent history of hospitalization in India. NDM-5 differed from existing enzymes due to substitutions at positions 88 (Val -> Leu) and 154 (Met -> Leu) and reduced the susceptibility of E. coli TOP10 transformants to expanded-spectrum cephalosporins and carbapenems when expressed under its native promoter.