Characterization of the subcellular localization and nuclear import molecular mechanisms of herpes simplex virus 1 UL2
Characterization of the subcellular localization and nuclear import molecular mechanisms of herpes simplex virus 1 UL2
复制标题
单纯疱疹病毒1 UL2亚细胞定位和入核分子机制的表征
DOI：
10.1515/hsz-2016-0268
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发表时间：
2017-04-01
影响因子：
3.7
通讯作者：
Li, Meili
中科院分区：
文献类型：
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作者：
Cai, Mingsheng;Huang, Zebin;Li, Meili
摘要单纯疱疹病毒1型（HSV-1）UL 2蛋白作为一种重要的蛋白质，参与了病毒感染的各个阶段，但其确切的亚细胞定位和转运分子决定簇至今仍不清楚。在本研究中，通过使用活细胞荧光显微镜分析，增强型黄色荧光蛋白标记的UL 2在活细胞中瞬时表达，并显示完全核积累，而不存在其他HSV-1蛋白。此外，UL 2的核转运是通过Ran-、importin α1-、α5-、α7-、β1-和transportin-1细胞转运受体的多种转运途径进行的。因此，这些结果将提高对HSV-1感染周期中UL 2介导的生物学功能的理解。
Abstract As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay, UL2 tagged with enhanced yellow fluorescent protein was transiently expressed in live cells and showed a completely nuclear accumulation without the presence of other HSV-1 proteins. Moreover, the nuclear transport of UL2 was characterized to be assisted by multiple transport pathways through Ran-, importin α1-, α5-, α7-, β1- and transportin-1 cellular transport receptors. Consequently, these results will improve understanding of UL2-mediated biological functions in HSV-1 infection cycles.