Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose.
Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose.
复制标题
DNA 从聚丙烯酰胺或琼脂糖凝胶到硝化纤维素的定量电泳转移。
DOI：
10.1016/0003-2697(84)90356-7
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发表时间：
1984
影响因子：
2.9
通讯作者：
Lieberman,MW
中科院分区：
文献类型：
--
作者：
Smith,MR;Devine,CS;Cohn,SM;Lieberman,MW
开发了一种将 DNA 片段从聚丙烯酰胺凝胶高效电泳转移至硝酸纤维素片的方法。可以通过标准技术进行与这些片段的杂交。该方法还适用于琼脂糖凝胶，因此该转移方法可用于 40 至至少 23,000 bp 范围内的 DNA。
A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.