Antiviral activities of hybrids of two major human leukocyte interferons.
Antiviral activities of hybrids of two major human leukocyte interferons.
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两种主要人类白细胞干扰素杂交体的抗病毒活性。
DOI：
10.1093/nar/9.22.6153
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发表时间：
1981
影响因子：
14.9
通讯作者：
D. Goeddel
中科院分区：
文献类型：
--
作者：
Phillip K. Weck;S. Apperson;Nowell Stebbing;Patrick W. Gray;David W. Leung;H. Shepard;D. Goeddel
通过 LeIF-A (IFN-α 2) 和 LeIF-D (IFN-α 1) 基因在其编码区内常见限制性内切酶位点的体外重组，构建了四种杂交人白细胞干扰素 (LeIF 或 IFN-α) 基因。这些杂合基因已在trp启动子控制下在大肠杆菌中表达。产生的干扰素 [LeIF-AD (BglII)、-AD (PvuII)、-DA (BglII)、-DA (PvuII)] 具有与亲本分子 LeIF-A 和 -D 不同的抗病毒特性，其抑制不同病毒在一系列哺乳动物细胞中形成噬斑的能力差异很大。所有六个克隆的 LeIF 均表现出天然白细胞干扰素的热稳定性、pH 2 稳定性和抗原特异性。
Four hybrid human leukocyte interferon (LeIF or IFN-alpha) genes have been constructed by in vitro recombination of LeIF-A (IFN-alpha 2) and LeIF-D (IFN-alpha 1) genes at common restriction endonuclease sites located within their coding regions. These hybrid genes have been expressed in E. coli under trp promoter control. The interferons produced [LeIF-AD (BglII), -AD (PvuII), -DA (BglII), -DA (PvuII)] have antiviral properties distinct from the parental molecules LeIF-A and -D, varying considerably in their abilities to inhibit plaque formation by different viruses in a range of mammalian cells. All six of the cloned LeIFs exhibit the heat stability, pH 2 stability and antigenic specificity of natural leukocyte interferons.