EFFECT OF EXCESS DIETARY RETINOIC ACID ON SKIN PAPILLOMA AND CARCINOMA FORMATION INDUCED BY A COMPLETE CARCINOGENESIS PROTOCOL IN FEMALE SENCAR MICE
EFFECT OF EXCESS DIETARY RETINOIC ACID ON SKIN PAPILLOMA AND CARCINOMA FORMATION INDUCED BY A COMPLETE CARCINOGENESIS PROTOCOL IN FEMALE SENCAR MICE
复制标题
DOI：
10.1016/0304-3835(94)90032-9
复制
发表时间：
1994-04-01
期刊：
影响因子：
9.7
通讯作者：
DE LUCA, LM
中科院分区：
文献类型：
--
作者：
CHEN, LC;KIRCHHOFF, S;DE LUCA, LM
以前，我们已经证明，在药理剂量(30微克/克饮食)的饮食维甲酸(RA)抑制皮肤乳头状瘤向癌变的两阶段致癌方案，以7，12-二甲基苯并[a]菲(DMBA)为引发剂，12-O-十四烷基佛波醇-13-乙酸酯(TPA)为启动子(De Luca等人，Carcinationology，14(1993)539-542)。本研究的目的是确定饮食中维甲酸对雌性Sencar小鼠皮肤乳头状瘤和癌症形成的影响。3周龄的小鼠断奶后，饲喂含有3(对照组)或30(过量)mU/g RA的饲料，并外用DMBA(25.5 mU g)，每周一次，持续20周。在体重、存活率、乳头状瘤发生率、累积癌症发生率(19.4%比23.7%)、癌产率(0.19比0.26/只小鼠)、肿瘤转化率(5.2%比3.9%)和平均癌变年龄(22.7±4.7岁比23.3±2.8周)方面，过量饮食RA组小鼠与对照组小鼠没有显著差异。然而，乳头状瘤的产率下降了约50%(即在第20周时为3.7比7.0，P
Previously, we have shown that dietary retinoic acid (RA) at pharmacological doses (30 mu g/g of diet) inhibited the malignant conversion of skin papillomas to carcinomas induced by a two-stage carcinogenesis protocol with 7,12-dimethylbenz[a]anthracene (DMBA) as initiator and 12-O-tetradecanoylphorbol-13-acetate (TPA) as promoter (De Luca et al., Carcinogenesis, 14 (1993) 539-542). The purpose of this study was to determine the effect of dietary RA on skin papilloma and carcinoma formation induced by a complete carcinogenesis protocol with repeated DMBA treatment in female Sencar mice. Mice at 3 weeks of age were weaned onto a diet containing either 3 (control) or 30 (excess) mu g of RA/g of diet and treated topically with DMBA (25.5 mu g) once per week for 20 weeks. Mice fed excess dietary RA did not significantly differ from control mice in the following parameters: body weight, survival rate, papilloma incidence, cumulative carcinoma incidence (19.4% versus 23.7%), carcinoma yield (0.19 versus 0.26 per mouse), carcinoma conversion efficiency (5.2% versus 3.9%), and average age of carcinoma development (22.7 +/- 4.7 versus 23.3 +/- 2.8 weeks). However, papilloma yield was decreased by about 50% (i.e. 3.7 versus 7.0 at week 20, P