Methylene blue decreases ischemia-reperfusion (I/R)-induced spinal cord injury: An in vivo study in an I/R rabbit model
Methylene blue decreases ischemia-reperfusion (I/R)-induced spinal cord injury: An in vivo study in an I/R rabbit model
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DOI：
10.1159/000096007
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发表时间：
2006-01-01
影响因子：
1.6
通讯作者：
Cobanoglu, A.
中科院分区：
文献类型：
--
作者：
Bardakci, H.;Kaplan, S.;Cobanoglu, A.
目的：探讨静脉注射亚甲蓝（MB）对脊髓缺血再灌注（I/R）损伤的影响。研究方法：16只家兔随机分为M组（n = 8;接受MB，干预组）或C组（n = 8;对照组），并通过夹闭左肾动脉和主动脉分叉之间的腹主动脉进行30分钟的SC缺血。钳夹前15 min，M组静脉注射MB（10 mg/kg），C组静脉注射生理盐水。术后24小时，使用Tarlov评分对两组进行组织学和神经功能比较。还进行了测定SC组织中丙二醛（MDA）和谷胱甘肽（GSH）水平的测量。结果：MB治疗组动物的神经功能损害和脊髓组织MDA水平显著降低（p < 0.001）。相反，脊髓GSH水平在M组中显著较高（p < 0.001）。组织学检查显示，MB组SC的完整性得到更好的保护，而对照组的脊髓显示出急性神经元损伤的证据。结论：预防性使用MB可减少兔SC I/R模型中的神经损伤并改善临床结局。这些作用可能是由药物的抗氧化特性介导的。版权所有（c）2006 S. Karger AG，巴塞尔。
Objectives: To evaluate the effects of intravenous methylene blue (MB) administration on ischemia-reperfusion (I/R) injury of the spinal cord (SC). Methods: 16 rabbits were randomly assigned either to group M (n = 8; receiving MB, intervention group) or group C (n = 8; control group) and underwent a 30-min period of SC ischemia by clamping the abdominal aorta between the left renal artery and the aortic bifurcation. 15 min before clamping, rabbits received either intravenous MB (10 mg/kg; group M) or normal saline (group C). The two groups were compared 24 h postoperatively both histologically and for neurological function, using a Tarlov score. Measurements to determine levels of malondialdehyde (MDA) and glutathione (GSH) in the SC tissue were also performed. Results: Neurological impairment and spinal tissue MDA levels were significantly lower in animals treated with MB (p < 0.001). In contrast, spinal GSH levels were significantly higher in group M (p < 0.001). Histological examination revealed that the integrity of the SC was better preserved in the MB group, whereas cords from the control group exhibited evidence of acute neuronal injury. Conclusions: The prophylactic use of MB reduces neurological injury and improves clinical outcomes in the rabbit SC I/R model. These effects are probably mediated by the drug's antioxidant properties. Copyright (c) 2006 S. Karger AG, Basel.