Evidence for an interplay between cell cycle progression and the initiation of differentiation between life cycle forms of African trypanosomes.
Evidence for an interplay between cell cycle progression and the initiation of differentiation between life cycle forms of African trypanosomes.
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DOI：
10.1083/jcb.125.5.1147
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发表时间：
1994-06
期刊：
影响因子：
--
通讯作者：
Gull K
中科院分区：
文献类型：
--
作者：
Matthews KR;Gull K
非洲锥虫在哺乳动物宿主血流和采采蝇载体之间的成功传播涉及寄生虫形态和生物化学的显著改变。这种分化通过舌蝇中肠原循环形式伴随着重新进入增殖细胞周期。使用一个同步分化模型和各种标志物诊断通过分化和细胞周期的进展，我们已经调查了这两个过程之间的相互作用。我们的研究结果暗示锥虫细胞周期的位置和分化信号的感知之间的关系，并表明不可逆的承诺分化后迅速发生诱导。此外，我们表明，分化群体中重新进入细胞周期是同步的，并且一旦启动，分化途径的进展就可以与细胞周期的进展脱钩。
Successful transmission of the African trypanosome between the mammalian host blood-stream and the tsetse fly vector involves dramatic alterations in the parasite's morphology and biochemistry. This differentiation through to the tsetse midgut procyclic form is accompanied by re-entry into a proliferative cell cycle. Using a synchronous differentiation model and a variety of markers diagnostic for progress through both differentiation and the cell cycle, we have investigated the interplay between these two processes. Our results implicate a relationship between the trypanosome cell cycle position and the perception of the differentiation signal and demonstrate that irreversible commitment to the differentiation occurs rapidly after induction. Furthermore, we show that re-entry into the cell cycle in the differentiating population is synchronous, and that once initiated, progress through the differentiation pathway can be uncoupled from progress through the cell cycle.