Progesterone Treatment Shows Benefit in Female Rats in a Pediatric Model of Controlled Cortical Impact Injury.
Progesterone Treatment Shows Benefit in Female Rats in a Pediatric Model of Controlled Cortical Impact Injury.
复制标题
DOI：
10.1371/journal.pone.0146419
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Sayeed I
中科院分区：
文献类型：
--
作者：
Geddes RI;Peterson BL;Stein DG;Sayeed I
我们最近的研究表明，黄体酮治疗可以减少未成熟雄性大鼠中至重度双侧内侧前额皮质损伤后的病变大小和行为缺陷。在非常年轻的受试者中，对损伤和黄体酮治疗的反应是否存在重要的性别差异尚未得到足够的重视。在这里，我们研究了黄体酮在相同的脑损伤模型中对青春期前雌性动物的影响。28日龄雌性Sprague-Dawley大鼠接受假性（n = 14）或控制性皮质冲击（CCI）损伤（n = 21），于伤后1-7天（PID）给予孕酮（8 mg/kg体重）或载体注射，并于PID 9-27进行行为学测试。在PID 28时评估脑损伤大小。车辆处理的CCI损伤雌性大鼠的病变大小比先前报道的同样处理的年龄匹配的雄性大鼠小。孕酮治疗降低了CCI对损伤程度和行为缺陷的影响。孕激素治疗后，前额皮质中线CCI损伤的青春期前雌性大鼠形态和功能缺陷减少。虽然观察到对这种损伤的易感性存在性别差异，但在CCI后的雌雄幼鼠中，黄体酮治疗都产生了有益的效果。
We recently showed that progesterone treatment can reduce lesion size and behavioral deficits after moderate-to-severe bilateral injury to the medial prefrontal cortex in immature male rats. Whether there are important sex differences in response to injury and progesterone treatment in very young subjects has not been given sufficient attention. Here we investigated progesterone’s effects in the same model of brain injury but with pre-pubescent females. Twenty-eight-day-old female Sprague-Dawley rats received sham (n = 14) or controlled cortical impact (CCI) (n = 21) injury, were given progesterone (8 mg/kg body weight) or vehicle injections on post-injury days (PID) 1–7, and underwent behavioral testing from PID 9–27. Brains were evaluated for lesion size at PID 28. Lesion size in vehicle-treated female rats with CCI injury was smaller than that previously reported for similarly treated age-matched male rats. Treatment with progesterone reduced the effect of CCI on extent of damage and behavioral deficits. Pre-pubescent female rats with midline CCI injury to the frontal cortex have reduced morphological and functional deficits following progesterone treatment. While gender differences in susceptibility to this injury were observed, progesterone treatment produced beneficial effects in young rats of both sexes following CCI.