On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers
On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers
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DOI：
10.1016/j.chroma.2011.05.062
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发表时间：
2011-07-22
影响因子：
4.1
通讯作者：
Pamme, Nicole
中科院分区：
文献类型：
--
作者：
De Leonardis, Francesco;Pascali, Giancarlo;Pamme, Nicole
微流体方法已经证明了对涉及正电子发射断层扫描（PET）核素的放射化学反应的相关影响，这是由于更短的反应时间和更小的前体量。然而，很少有人注意到放射性[F-18]氟化物离子的初始预浓缩和干燥的整合，这是标记放射性示踪剂化合物所需的。在这项工作中，我们报告的设计，制造和实施的玻璃微流体装置填充可回收的阴离子交换颗粒的重复回收[F-18]和[F-19]氟离子。该装置首先用非放射性[F-18]氟化物离子进行测试，结果表明，在40次连续实验运行中，它能重复捕获和去除>95%的氟化物，效率没有降低。然后，在20次实验中测试相同装置对[F-18]氟化物离子的捕获和释放，性能没有可测量的降低。最后，将[F-18]氟离子洗脱为（KF）-F-18/K2.2.2复合物，通过在乙腈中重复溶解并蒸发残余水来干燥，并与二甲苯磺酸乙酯（EtDT）反应，得到所需产物（[F-18]氟乙基甲苯磺酸酯），产率（RCY）为96 +/-3%。调节，捕获，洗脱和再生所需的总时间小于6分钟。这种方法将是一个集成的平台，能够进行更快，更安全的放射化学合成的微型非常有益的。(C)2011爱思唯尔有限公司版权所有。
Microfluidic approaches have demonstrated a relevant impact on radiochemical reactions involving Positron Emission Tomography (PET) nuclides, due to shorter reaction times and smaller precursor quantities. However, little attention has been given to the integration of the initial pre-concentration and drying of radioactive [F-18]fluoride ions, required for the labeling of radiotracer compounds. In this work we report the design, fabrication and implementation of a glass microfluidic device filled with recyclable anion exchange particles for the repeated recovery of [F-18] and [F-19]fluoride ions. The device was first tested with non radioactive [F-18]fluoride ions and it was shown to repeatedly trap and elute >95% fluoride over 40 successive experimental runs with no decrease in efficiency. The same device was then tested for the trapping and release of [F-18]fluoride ions over 20 experiments with no measurable decrease in performance. Finally, the [F-18]fluoride ions were eluted as a (KF)-F-18/K2.2.2 complex, dried by repeated dissolution in acetonitrile and evaporation of residual water, and reacted with ethyl ditosylate (EtDT) leading to the desired product ([F-18]fluoroethyltosylate) with 96 +/- 3% yield (RCY). The overall time needed for conditioning, trapping, elution and regeneration was less than 6 min. This approach will be of great benefit towards an integrated platform able to perform faster and safer radiochemical synthesis on the micro-scale. (C) 2011 Elsevier B.V. All rights reserved.