RNA-controlled assembly of tobacco mosaic virus-derived complex structures: from nanoboomerangs to tetrapods
RNA-controlled assembly of tobacco mosaic virus-derived complex structures: from nanoboomerangs to tetrapods
复制标题
DOI：
10.1039/c4nr05434b
复制
发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Wege, Christina
中科院分区：
文献类型：
--
作者：
Eber, Fabian J.;Eiben, Sabine;Wege, Christina
人工nanotubular核蛋白形状的基础上烟草花叶病毒（TMV）衍生的积木在体外组装产生不同的空间组织的病毒外壳蛋白亚基的基因工程RNA分子，含有两个或多个TMV装配起源（OA）。扭结的纳米回旋镖以及分支的多足动物的生长是由这些RNA决定的。一个很大程度上同时启动在两个起源和随后的双向管伸长可以通过透射电子显微镜观察的中间体和最终产品。新生管末端的碰撞产生了具有明确臂长的角状粒子。具有三到五个OA的RNA产生具有最多四个臂的分支多足动物。这种RNA指导的自组装的不常见的纳米管结构的复杂的多价纳米模板用于功能性杂化材料的制造的潜力进行了讨论。
The in vitro assembly of artificial nanotubular nucleoprotein shapes based on tobacco mosaic virus( TMV-)-derived building blocks yielded different spatial organizations of viral coat protein subunits on genetically engineered RNA molecules, containing two or multiple TMV origins of assembly (OAs). The growth of kinked nanoboomerangs as well as of branched multipods was determined by the encapsidated RNAs. A largely simultaneous initiation at two origins and subsequent bidirectional tube elongation could be visualized by transmission electron microscopy of intermediates and final products. Collision of the nascent tubes' ends produced angular particles with well-defined arm lengths. RNAs with three to five OAs generated branched multipods with a maximum of four arms. The potential of such an RNA-directed self-assembly of uncommon nanotubular architectures for the fabrication of complex multivalent nano-templates used in functional hybrid materials is discussed.