The glycosomes of trypanosomes: number and distribution as revealed by electron spectroscopic imaging and 3‐D reconstruction
The glycosomes of trypanosomes: number and distribution as revealed by electron spectroscopic imaging and 3‐D reconstruction
复制标题
锥虫糖体：电子光谱成像和 3D 重建显示的数量和分布
作者：
L. Tetley;K. Vickerman
在Zeiss 902电子显微镜上成像的0·35 μm厚切片的典型小体的计算机辅助三维重建被用于研究细胞组织的动力学。糖酵解酶在糖体中的分离引起了关于这些细胞器的分布和生物发生的问题。伊氏锥虫糖体的直接计数表明每个细胞有30-40个糖体，而与伊氏锥虫密切相关的T.布氏杆菌，每个细胞65个。这些数字与其他使用基于模型的形态测量方法获得每个细胞230个值的估计值形成对比。
Computer‐aided 3‐D reconstruction of typanosomes from 0·35‐μm‐thick sections imaged on the Zeiss 902 electron microscope are being used to study the dynamics of cell organization. Segregation of glycolytic enzymes into glycosomes raises questions concerning the distribution and biogenesis of these organelles. Direct counts of glycosomes from Trypanosoma evansi indicate 30–40 per cell and for the closely related T. brucei, 65 per cell. These figures contrast with the estimates of others who have used model‐based morphometric methods to obtain a value of 230 per cell.