Catalase activity in dogfish (Mustelus canis) ocular tissues.

Catalase activity in dogfish (Mustelus canis) ocular tissues.
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角鲨(Mustelus canis)眼组织中的过氧化氢酶活性。

DOI:
10.1086/bblv187n2p247
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发表时间:
1994
期刊:
The Biological bulletin
影响因子:
--
通讯作者:
Rafferty,NS
Rafferty,NS
中科院分区:
--
文献类型:
--
作者:
Zigman,S;Rafferty,NS

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综上所述,唇血红蛋白的分子量约为60000,通过PCMB滴定法测量,每个四聚体约有6个巯基,由两个不同的亚基组成。它被KsFe (CN) e或CuQ聚合成大约两到三个四聚体的聚合物。聚合被IAA抑制,被B-ME逆转,表明聚合可能是由于半胱氨酸巯基的氧化和分子间二硫键的形成。这项工作得到了PSC-CUNY补助金# 664308和665168以及NIGMS-NIH MARC计划补助金# 5T34GM-08 182的支持。
In conclusion, pout hemoglobin has a molecular weight of about 60,000, approximately six sulkydryl groups per tetramer, as measured by PCMB titration, and consists of two dissimilar subunits. It is polymerized, by KsFe (CN) e or CuQ, to polymers corresponding to about two to three tetramers. Polymerization is inhibited by IAA and reversed by B-ME, suggesting that polymerization may be due to the oxidation of cysteine sulthydryl groups and the formation of intermolecular disulfide bonds. This work has been supported by PSC-CUNY Grants# 664308 and 665168 and NIGMS-NIH MARC Program Grant# 5T34GM-08 182.