The complete mitochondrial genome sequence and gene organization of the mud crab (Scylla paramamosain) with phylogenetic consideration
The complete mitochondrial genome sequence and gene organization of the mud crab (Scylla paramamosain) with phylogenetic consideration
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泥蟹（Scylla paramamosain）完整的线粒体基因组序列和基因组织与系统发育的考虑
DOI：
10.1016/j.gene.2013.01.028
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发表时间：
2013-04-25
期刊：
影响因子：
3.5
通讯作者：
Ma, Lingbo
中科院分区：
文献类型：
--
作者：
Ma, Hongyu;Ma, Chunyan;Ma, Lingbo
完整的线粒体基因组对于更好地理解基因组水平的特征和近缘物种之间的系统发育关系具有重要意义。在本研究中，我们用454深度测序法和Sanger测序法测定了青蟹(Scylla Paramosain)的线粒体基因组DNA全序列。基因组DNA全长15,824个核苷酸，包括13个蛋白质编码基因、22个转运蛋白RNA(TRNA)基因、2个核糖体RNA(RRNA)基因和一个可能的控制区(CR)。在37个基因中，有23个由重链(H链)编码，其余由轻链(L链)编码。虽然tRNA(His)基因的相对位置与其他节肢动物不同，但线粒体基因组中的基因顺序与大多数节肢动物的基因顺序基本相同。在13个蛋白质编码基因中，有3个基因(ATP6、ND1和ND3)以罕见的起始密码子ATT开头，1个基因细胞色素C氧化酶亚基I(COI)以不完全终止密码子TA结尾。所有22个tRNA都可以折叠成典型的三叶草叶二级结构，基因大小在63-73bp之间。基于12个串联蛋白编码基因的系统发育分析表明，11属19个种的分子亲缘关系与传统分类学一致。(C)2013爱思唯尔B.V.保留所有权利。
The complete mitochondrial genome is of great importance for better understanding the genome-level characteristics and phylogenetic relationships among related species. In the present study, we determined the complete mitochondrial genome DNA sequence of the mud crab (Scylla paramamosain) by 454 deep sequencing and Sanger sequencing approaches. The complete genome DNA was 15,824 bp in length and contained a typical set of 13 protein-coding genes, 22 tran fer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and a putative control region (CR). Of 37 genes, twenty-three were encoded by the heavy strand (H-strand), while the other ones were encoded by light strand (L-strand). The gene order in the mitochondrial genome was largely identical to those obtained in most arthropods, although the relative position of gene tRNA(His) differed from other arthropods. Among 13 protein-coding genes, three (ATPase subunit 6 (ATP6), NADH dehydrogenase subunits 1 (ND1) and ND3) started with a rare start codon ATT, whereas, one gene cytochrome c oxidase subunit I (COI) ended with the incomplete stop codon TA. All 22 tRNAs could fold into a typical clover-leaf secondary structure, with the gene sizes ranging from 63 to 73 bp. The phylogenetic analysis based on 12 concatenated protein-coding genes showed that the molecular genetic relationship of 19 species of 11 genera was identical to the traditional taxonomy. (c) 2013 Elsevier B.V. All rights reserved.