Isolation and expression of two zebrafish homologues of parvalbumin genes related to chicken CPV3 and mammalian oncomodulin.
Isolation and expression of two zebrafish homologues of parvalbumin genes related to chicken CPV3 and mammalian oncomodulin.
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DOI：
10.1016/s0925-4773(02)00340-4
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发表时间：
2002-11-01
影响因子：
1.2
通讯作者：
Tsai, Huai-Jen
中科院分区：
文献类型：
--
作者：
Hsiao, Chung-Der;Tsai, Wei-Yuan;Tsai, Huai-Jen
从斑马鱼中分离到小白蛋白基因的两个β-型同源基因pvalb3a和pvalb3b的全长cDNA克隆。根据推导的氨基酸序列进行的同源性和系统发育分析表明，PVALB3A和PVALB3B是鸡CPV3和哺乳动物OCM的同源同源物，但与四足动物的阿尔法型PVALB和骨鱼的肌肉型PVALB不同。整体原位杂交结果表明，pvalb3a和pvalb3b在胚胎发育早期的时空表达具有明显的差异性和高度的发育调节性。与鸡的CPV3和哺乳动物的OCM不同，斑马鱼的pvalb3a转录本广泛表达在粘液细胞、嗅觉上皮、垂体前叶、咽齿胚、巨噬细胞、内耳和侧线神经肥大细胞中，而pvalb3b转录本在卵黄合胞层、内耳和前肾导管中的表达更为有限。
Full-length cDNA clones coded for two beta-type homologues of parvalbumin genes, pvalb3a and pvalb3b, were isolated from zebrafish. The homology and phylogenetic analyses, based on the deduced amino acid sequences, revealed that PVALB3A and PVALB3B are co-orthologues to chicken CPV3 and mammalian oncomodulin (OCM) but are divergent from alpha-type PVALB of tetrapods and muscle-type PVALB of bony fish. Whole-mount in situ hybridization revealed that the spatio-temporal expression of pvalb3a and pvalb3b were distinct and highly development-regulated during early embryogenesis. Unlike their counterparts of CPV3 in chicken and OCM in mammals, zebrafish pvalb3a transcripts were widely expressed in mucous cells, the olfactory epithelium, anterior pituitary, pharyngeal teeth germ, macrophages, inner ear and lateral line neuromasts, whereas, pvalb3b transcripts were more restrictedly expressed in the yolk syncytial layer, inner ear and pronephric ducts.