Sequence and expression analysis of the hrpB pathogenicity operon of Xanthomonas campestris pv vesicatoria which encodes eight proteins with similarity to components of the Hrp, Ysc, Spa, and Fli secretion systems
Sequence and expression analysis of the hrpB pathogenicity operon of Xanthomonas campestris pv vesicatoria which encodes eight proteins with similarity to components of the Hrp, Ysc, Spa, and Fli secretion systems
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DOI：
10.1094/mpmi-8-0845
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发表时间：
1995-11-01
影响因子：
3.5
通讯作者：
Bonas, U
中科院分区：
文献类型：
--
作者：
Fenselau, S;Bonas, U
本文描述了野油菜黄单胞菌中最大操纵子hrpB的分子特征。Vesicatoria HRP簇。HrpB区全长6kb，编码8种蛋白，其中7种在大肠杆菌中表达。HrpB3蛋白是唯一一个在N端携带信号肽序列的蛋白，是一种可能定位于野油菜黄单胞菌外膜的脂蛋白。口水症。HrpB4和HrpB8蛋白分别含有一个和五个假定的跨膜结构域，很可能与内膜有关。HrpB3、HrpB5、HrpB6和HrpB8蛋白与细菌中不同III型蛋白分泌途径的可能成分具有序列相似性。例子包括来自其他植物病原体的HRP蛋白，耶尔森氏菌的YscJ、YscN、YscL和YscT，以及福氏志贺氏菌的MxiJ、Spa47和Spa29。鉴定了转录起始点和hrpB启动子。最小的hrpB启动子区域包含一个新的序列基序PIP-box，它可能在hrpB操纵子的转录激活中发挥作用，也可能在植物诱导的其他野生型油菜夜蛾基因的转录激活中发挥作用。口水症。
In this paper we describe the molecular characterization of hrpB, the largest operon in the Xanthomonas campestris pv. vesicatoria hrp cluster. The hrpB region encompasses 6 kb and encodes eight putative proteins, seven of which were expressed in Escherichia coli. The HrpB3 protein is the only one carrying a signal peptide sequence at the N-terminus and is a putative lipoprotein localized in the outer membrane of X. campestris pv. vesicatoria. The HrpB4 and HrpB8 proteins contain one and five putative transmembrane domains, respectively, and are most likely associated with the inner membrane. The HrpB3, HrpB5, HrpB6, and HrpB8 proteins show sequence similarity to putative components of different type III protein secretion pathways in bacteria. Examples include Hrp proteins from other plant pathogens, YscJ, YscN, YscL, and YscT of Yersinia spp., and MxiJ, Spa47, and Spa29 of Shigella flexneri. The transcription start site and the hrpB promoter was identified. The minimal hrpB promoter region of 90 bp contains a novel sequence motif, the PIP-box, which might play a role in transcription activation of the hrpB operon and possibly other plant-induced genes of X. campestris pv. vesicatoria.