Growth and characterization of fibroblasts obtained from bronchoalveolar lavage of patients with scleroderma.
Growth and characterization of fibroblasts obtained from bronchoalveolar lavage of patients with scleroderma.
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发表时间：
1992-11
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通讯作者：
A. Ludwicka;M. Trojanowska;E. Smith;M. Baumann;C. Strange;J. Korn;T. Smith;E. Leroy;R. Silver
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作者：
A. Ludwicka;M. Trojanowska;E. Smith;M. Baumann;C. Strange;J. Korn;T. Smith;E. Leroy;R. Silver
我们描述了从53%的硬皮病患者的支气管肺泡灌洗液（BAL）中检测和生长具有人类平滑肌细胞分化特征的成纤维细胞，而不是从健康对照中。硬皮病肺成纤维细胞对α -actin、vimentin和desmin抗体的结合超过正常成人肺成纤维细胞，表明硬皮病肺成纤维细胞表达了一些人平滑肌细胞分化（肌成纤维细胞）的标志物，这可能是生物学行为差异的原因。mRNA分析证实了间充质细胞表型，在这些细胞中高表达I型胶原和纤维连接蛋白。硬皮病肺泡巨噬细胞释放的纤维连接蛋白也明显高于健康供者的巨噬细胞。
We describe the detection and the growth of fibroblasts with human smooth muscle cell differentiation features from bronchoalveolar lavage (BAL) fluid of 53% of patients with scleroderma, but not from healthy controls. The binding of alpha-actin, vimentin and desmin antibodies by scleroderma lung fibroblasts exceeded that of normal adult lung fibroblasts, indicating that scleroderma lung fibroblasts express some markers of human smooth muscle cell differentiation (myofibroblasts), which may account for differences in biological behavior. A mesenchymal cell phenotype was documented by mRNA analysis, showing high expression of collagen type I and fibronectin in these cells. Fibronectin is also released in significantly higher amounts by scleroderma alveolar macrophages than by macrophages from healthy donors.