Higher sensitivity of male CD4+ T cells to suppressive effects of CD8+ T cells on IL-5 production compared to female CD4+ T cells
Higher sensitivity of male CD4+ T cells to suppressive effects of CD8+ T cells on IL-5 production compared to female CD4+ T cells
复制标题
与女性 CD4 T 细胞相比，男性 CD4 T 细胞对 CD8 T 细胞对 IL-5 产生的抑制作用具有更高的敏感性
DOI：
10.1159/000337759
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kaori Okuyama
中科院分区：
文献类型：
--
作者：
Kanehira M;Kikuchi T;Ohkouchi S;Shibahara T;Tode N;Santoso A;Daito H;Ohta H;Tamada T;Nukiwa T;Kaori Okuyama
研究背景青春期后女性哮喘患病率和严重程度高于男性。这种性别差异的潜在机制尚未完全了解。据报道，雌性小鼠中更严重的气道炎症与哮喘模型中更高水平的辅助性T细胞2型（Th 2）细胞因子相关。本研究的目的是探讨性别差异中的CD 4+和CD 8 + T细胞功能的Th 2细胞因子production. Methods脾细胞从幼稚小鼠与抗CD 3/CD 28抗体刺激和CD 4+和CD 8 + T细胞的比例进行了分析。在CD 8 + T细胞存在下刺激CD 4 + T细胞。结果雌性脾细胞中IL-5的浓度显著高于雄性脾细胞，而IFN-γ的浓度则无显著性差异。脾细胞中CD 4+和CD 8 + T细胞的比例无性别差异。尽管雄性和雌性CD 4 + T细胞中的IL-5产生水平相似，但雄性和雌性CD 8 + T细胞均抑制雄性CD 4 + T细胞中的IL-5产生，而非雌性CD 4 + T细胞。而IL-5和IL-10均未在男性和女性的CD 8 + T细胞中检测到，女性CD 8 + T细胞中IFN-γ的浓度显著高于男性CD 8 + T细胞。
BackgroundAsthma prevalence and severity are higher in females than in males after puberty. The underlying mechanisms of this gender difference are not fully understood. More severe airway inflammation in female mice has been reported to be associated with higher levels of T helper type 2 (Th2) cytokines in asthma models. The aim of this study was to investigate sex differences in CD4+ and CD8+ T cell functions in Th2 cytokine production.MethodsSplenocytes from naive mice were stimulated with anti-CD3/CD28 antibodies and the proportions of CD4+ and CD8+ T cells were analyzed. CD4+ T cells were stimulated in the presence of CD8+ T cells. The concentrations of interleukin (IL)-5, IL-10 and interferon (IFN)-γ in the cultures were measured.ResultsThe concentration of IL-5, but not IFN-γ, was significantly higher in female splenocytes than in male splenocytes. There were no sex differences in the proportions of CD4+ and CD8+ T cells in the splenocytes. Although the IL-5 production levels in male and female CD4+ T cells were similar, IL-5 production in male CD4+ T cells, but not female CD4+ T cells, was suppressed by both male and female CD8+ T cells. While IL-5 and IL-10 were not detected in the cultures from both male and female CD8+ T cells, IFN-γ concentration in female CD8+ T cells was significantly higher than in male CD8+ T cells.ConclusionsThe sex difference in the sensitivity of CD4+ T cells to CD8+ T cell suppression might contribute to the sex difference in IL-5 production by splenocytes.