Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis.
Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis.
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DOI：
10.1094/mpmi-18-0385
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
Yanqing Wang;Yumiko Ohara;H. Nakayashiki;Y. Tosa;S. Mayama
中科院分区：
文献类型：
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作者：
Yanqing Wang;Yumiko Ohara;H. Nakayashiki;Y. Tosa;S. Mayama
荧光假单胞菌（Pseudomonas fluorescens） FPT9601-T5最初被鉴定为番茄内生植物促生长根瘤菌（PGPR）。为了对荧光假单胞菌FPT9601-T5定殖引发的寄主生理生化变化进行分子解剖，本研究以模式植物拟南芥为研究对象。用荧光假单胞菌FPT9601-T5定殖拟南芥，可在接种后3周后促进植株生长，部分抑制丁香假单胞菌pv引起的疾病症状。表明荧光假单胞菌FPT9601-T5在拟南芥中发挥了PGPR的作用。为了获得拟南芥- fpt9601 - t5相互作用过程中转录修饰的全局视图，我们使用Affymetrix基因芯片探针阵列进行了微阵列分析，其中包含大约22,800个基因。结果表明，与对照相比，fpt9601 - t5处理的拟南芥中有95个和105个基因分别上调或下调了2倍以上。上调的基因包括代谢、信号转导和应激反应。值得注意的是，FPT9601-T5定殖后，生长素调控基因和结节样基因上调，一些乙烯应答基因下调。我们的研究结果表明，荧光假单胞菌FPT9601-T5以类似于已知PGPR的方式触发植物反应，至少在某些方面，对根瘤菌有类似的反应。
Pseudomonas fluorescens FPT9601-T5 was originally identified as an endophytic plant growth-promoting rhizobacteria (PGPR) on tomato. To perform a molecular dissecttion of physiological and biochemical changes occurring in the host triggered by P. fluorescens FPT9601-T5 colonization, the model plant Arabidopsis was used in this study. Root colonization of Arabidopsis with P. fluorescens FPT9601-T5 promoted plant growth later than three weeks after inoculation and partially suppressed disease symptoms caused by Pseudomonas syringae pv. tomato DC3000, indicating that P. fluorescens FPT9601-T5 acted as a PGPR on Arabidopsis. To obtain a global view on transcript modification during the Arabidopsis-FPT9601-T5 interaction, we performed microarray analysis using Affymetrix Genechip probe arrays representing approximately 22,800 genes. The results showed that 95 and 105 genes were up- or down-regulated, respectively, more than twofold in FPT9601-T5-treated Arabidopsis plants as compared with control plants. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Noteworthy, upon FPT9601-T5 colonization, putative auxin-regulated genes and nodulin-like genes were up-regulated, and some ethylene-responsive genes were down-regulated. Our results suggest that P. fluorescens FPT9601-T5 triggered plant responses in a manner similar to known PGPR and, at least in some aspects, to rhizobia.