R-Mix cells are faster, at least as sensitive and marginally more costly than conventional cell lines for the detection of respiratory viruses
R-Mix cells are faster, at least as sensitive and marginally more costly than conventional cell lines for the detection of respiratory viruses
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DOI：
10.1016/s1386-6532(01)00171-8
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发表时间：
2001-08-01
影响因子：
8.8
通讯作者：
Guttman, K
中科院分区：
文献类型：
--
作者：
Barenfanger, J;Drake, C;Guttman, K
目的：评价水貂肺细胞与人腺癌细胞（分别为Mv1Lu和A549株，Diagnostic Hybrids, Athens， OH）的组合壳瓶R-Mix在临床呼吸道标本和冷冻储备中检测呼吸道病毒的能力。研究设计：我们比较了R-Mix与常规培养（CC）的性能，使用PMK、Hep-2和MRC5管检测新鲜临床标本中的呼吸道病毒。对于提交病毒学的每个呼吸道标本，接种两个壳瓶R-Mix并进行两次间接检测（通常在24和48小时之后），采用一种针对甲型流感和乙型流感、腺病毒、副流感1-3和RSV的免疫荧光抗血清池进行检测（DAKO, Carpinteria， CA）。如果阳性，则进行单克隆抗血清检测。cc孵育10天，每天检查细胞病理学效果，2次吸附，阳性染色。进行了成本比较。最后，将前几年冷冻的呼吸道病毒接种到R-Mix上。结果：29份冷冻病毒库R-Mix均呈阳性。在临床试验中，396例前瞻性标本接种于R-Mix和CC， R-Mix鉴定21例呼吸道病毒阳性；CC确定了19个。阳性标本R-Mix周转时间为1.4 d；CC为5.2天。所有标本（阳性和阴性）R-Mix的周转时间为2.0 d；CC为9.8天。结论：R-Mix能快速鉴定所有代表7种主要呼吸道病毒群的冷冻病毒库。与CC相比，R-Mix比CC中使用的三种细胞系（四管）略敏感，但快了几天。(C) 2001 Elsevier Science B.V.版权所有
Objective: To evaluate shell vials of R-Mix, a combination of mink lung cells and human adenocarcinoma cells (strains Mv1Lu and A549, respectively, Diagnostic Hybrids, Athens, OH) to detect respiratory viruses from prospective clinical respiratory specimens and frozen stocks. Study design: We compared the performance of R-Mix to conventional culture (CC) using tubes of PMK, Hep-2, and MRC5 to detect respiratory viruses from fresh clinical specimens. For each respiratory specimen submitted to virology, two shell vials of R-Mix were inoculated and examined twice (generally after 24 and 48 h) by an indirect test with a pool of immunofluorescent antisera to influenza A and B, adenovirus, parainfluenza 1-3 and RSV (DAKO, Carpinteria, CA). If positive, testing with monoclonal antisera was done. CCs were incubated for 10 days, examined daily for cytopathological effect, hemadsorbed twice and stained if positive. Cost comparison was done. Lastly, respiratory viruses frozen from previous years were inoculated onto R-Mix. Results: R-Mix was positive for all 29 frozen virus stocks. In the clinical trial, 396 prospective specimens were inoculated into R-Mix and CC. R-Mix identified 21 specimens as respiratory virus positive; CC identified 19. Turn-around time of R-Mix for positive specimens was 1.4 days; for CC it was 5.2 days. Turn-around time of R-Mix for all specimens (positive and negative) was 2.0 days; for CC it was 9.8 days. The overall cost of R-Mix was similar to 11% more than that of CC. Conclusion: R-Mix enabled rapid identification of all the frozen virus stocks representing the seven major respiratory viral groups. When compared to CC, R-Mix was slightly more sensitive than three cell lines (four tubes) used in CC but it was several days faster. (C) 2001 Elsevier Science B.V. All rights reserved.