Effect of feeding a high fat diet on hydrogen sulfide (H2S) metabolism in the mouse
Effect of feeding a high fat diet on hydrogen sulfide (H2S) metabolism in the mouse
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DOI：
10.1016/j.niox.2014.03.002
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发表时间：
2014-09-15
影响因子：
3.9
通讯作者：
Moore, Philip K.
中科院分区：
文献类型：
--
作者：
Peh, Meng Teng;Anwar, Azzahra Binti;Moore, Philip K.
硫化氢（H2S）在炎症中具有复杂的作用，据报道这种气体具有促炎和抗炎作用。最近的研究表明，缺乏H2S发生在，并可能有助于，慢性炎症的基础正在进行的动脉粥样硬化疾病。然而，是否高脂肪饮食，易患动脉粥样硬化，影响硫化氢代谢尚不清楚。在这项研究中，我们评估了H2S代谢不同组织的小鼠喂养高脂肪饮食长达16周。H2S的离体生物合成在高脂肪喂养的小鼠的肝、肾和肺中减少。Western blotting显示肝和肺中胱硫醚γ裂解酶（CSE）缺乏，肝和肾中胱硫醚β合成酶（CBS）表达增加。3-巯基丙酮酸硫转移酶（3-MST）的表达在肝脏中减少，但在其他组织中不减少。经化学方法测定，高脂喂养动物的主动脉内皮细胞CSE也降低。这些动物的血浆H2S浓度没有变化。在高脂喂养动物的动脉中没有明显的脂质沉积证据，血浆血清淀粉样蛋白A（SAA）和C-反应蛋白（CRP）也没有变化，表明没有明显的动脉粥样硬化疾病。血浆IL-6、IL 12 p40和G-CSF水平通过高脂肪喂养而增加，而其他细胞因子包括IL-1 α、IL-1b和TNF-α没有改变。这些结果表明，组织CSE和H2S的缺乏发生在喂食高脂肪饮食的小鼠中，并且这种变化发生在坦率的动脉粥样硬化疾病的发展之前。(C)2014爱思唯尔公司All rights reserved.
Hydrogen sulfide (H2S) has complex effects in inflammation with both pro- and anti-inflammatory actions of this gas reported. Recent work suggests that a deficiency of H2S occurs in, and may contribute to, the chronic inflammation which underpins ongoing atherosclerotic disease. However, whether a high fat diet, predisposing to atherosclerosis, affects H2S metabolism is not known. In this study we assessed H2S metabolism in different tissues of mice fed a high fat diet for up to 16 weeks. Ex vivo biosynthesis of H2S was reduced in liver, kidney and lung of high fat fed mice. Western blotting revealed deficiency of cystathionine gamma lyase (CSE) in liver and lung with increased expression of cystathionine beta synthetase (CBS) in liver and kidney. Expression of 3-mercaptopyruvate sulfurtransferase (3-MST) was reduced in liver but not other tissues. Aortic endothelial cell CSE was also reduced in high fat fed animals as determined immunohistochemically. Plasma H2S concentration was not changed in these animals. No evidence of lipid deposition was apparent in aortae from high fat fed animals and plasma serum amyloid A (SAA) and C-reactive protein (CRP) were also unchanged suggesting lack of frank atherosclerotic disease. Plasma IL-6, IL12p40 and G-CSF levels were increased by high fat feeding whilst other cytokines including IL-1 alpha, IL-lb and TNF-alpha were not altered. These results suggest that deficiency of tissue CSE and H2S occurs in mice fed a high fat diet and that this change takes place prior to development of frank atherosclerotic disease. (C) 2014 Elsevier Inc. All rights reserved.