Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging
Time-Resolved Digital Immunoassay for Rapid and Sensitive Quantitation of Procalcitonin with Plasmonic Imaging
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DOI：
10.1021/acsnano.9b02771
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发表时间：
2019-08-01
期刊：
影响因子：
17.1
通讯作者：
Tao, Nongjian
中科院分区：
文献类型：
--
作者：
Jing, Wenwen;Wang, Yan;Tao, Nongjian
及时诊断急性疾病可以改善治疗效果并挽救生命，但需要快速精确地量化生物标志物。在这里，我们报告了一种基于等离子体成像的时间分辨数字免疫测定，该等离子体成像将单个纳米颗粒与传感器表面上捕获的生物标志物结合。等离子体成像的实时和高对比度导致在宽动态范围内对个体生物标志物进行快速和精确的计数。我们证明了检测原理，以降钙素原（PCT）为例评价了该方法的性能，并实现了检测限为2.8 pg/mL，动态范围为4.2-12500 pg/mL，总检测时间为25 min。
Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of similar to 2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of similar to 25 min.