Isolation, characterization, and bioactivities of compounds from Fuscoporia torulosa mushroom
Isolation, characterization, and bioactivities of compounds from Fuscoporia torulosa mushroom
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DOI：
10.1111/jfbc.13074
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发表时间：
2019-12-01
影响因子：
4
通讯作者：
Ozturk, Mehmet
中科院分区：
文献类型：
--
作者：
Deveci, Ebru;Tel-Cayan, Gulsen;Ozturk, Mehmet
对Fuscoporia torulosa提取物进行色谱纯化，分离并鉴定了一种新的类固醇，5 α，8 α-epidioxyergosta-6，22-dien-3 beta-il-palmitate（1）和10种已知化合物（2-11）。化合物的结构经IR、NMR、MS分析及与文献数据比较确定。对MCF-7（乳腺癌），PC-3（前列腺癌），和3 T3（非肿瘤）的提取物和细胞毒性，抗氧化剂，胆碱酯酶，酪氨酸酶抑制活性的所有分离的化合物的细胞毒性活性进行了评价。甲醇提取物和化合物8显示出对MCF-7的最佳细胞毒性，而己烷提取物和化合物4显示出对PC-3的最高细胞毒性。化合物10和11显示出比α-生育酚和丁基化羟基茴香醚（BHA）更高的抗氧化活性，所述α-生育酚和丁基化羟基茴香醚（BHA）在ABTS（中心点+）、DPPH中心点和铜还原抗氧化能力（CUPRAC）测定中用作标准。此外，对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的胆碱酯酶抑制活性，化合物4和8被确定为最具活性的化合物。在所有分离的化合物中，化合物11表现出最高的酪氨酸酶抑制活性。
Chromatographic purification of Fuscoporia torulosa extracts resulted in the isolation and characterization of a new steroid, 5 alpha,8 alpha-epidioxyergosta-6,22-dien-3 beta-il-palmitate (1) and 10 known compounds (2-11). The structures of compounds were elucidated by IR, NMR, MS analyses, and comparison with literature data. Cytotoxic activities against MCF-7 (breast cancer), PC-3 (prostate cancer), and 3T3 (nontumor) of the extracts and cytotoxic, antioxidant, cholinesterase, and tyrosinase inhibitory activities of all isolated compounds were evaluated. The methanol extract and Compound 8 showed the best cytotoxicity against MCF-7, whereas the hexane extract and Compound 4 displayed the highest cytotoxicity against PC-3. Compounds 10 and 11 displayed higher antioxidant activity than alpha-tocopherol and butylated hydroxyanisole (BHA) which are used as standards in ABTS(center dot+), DPPH center dot, and cupric reducing antioxidant capacity (CUPRAC) assays. Also, cholinesterase inhibitory activity against acetylcholinesterase (AChE) and butrylcholinesterase (BChE), Compounds 4 and 8 were determined as the most active compounds. Among all isolated compounds, Compound 11 exhibited the highest tyrosinase inhibitory activity.