Hierarchical and Dynamic Regulation of Defense-Responsive Specialized Metabolism by WRKY and MYB Transcription Factors
Hierarchical and Dynamic Regulation of Defense-Responsive Specialized Metabolism by WRKY and MYB Transcription Factors
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DOI：
10.3389/fpls.2019.01775
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发表时间：
2020-01-31
影响因子：
5.6
通讯作者：
Clay, Nicole K.
中科院分区：
文献类型：
--
作者：
Barco, Brenden;Clay, Nicole K.
植物界产生数十万种特殊的生物活性代谢物，其中一些具有制药和生物技术的重要性。它们的生物合成和功能研究已经进行了几十年，但对于功能重叠和调节活性不同的转录因子如何协调控制植物特化代谢的动力学和输出，人们知之甚少。在这里，我们对受病原体感染的完整寄主植物进行了转录因子干扰的时间研究。我们确定WRKY33是条件依赖的主调节剂，MYB51是分层基因网络中的双重功能调节剂，可能负责camalexin和4-羟基吲哚-3-羰基腈（4OH-ICN）途径中的基因表达动态和代谢通量。这个网络也可能促进了拟南芥中新进化的4OH-ICN通路被更保守的转录因子MYB51调控捕获。长期以来，人们一直认为植物特化代谢的可塑性和发育的渠道化应受到不同的调控；我们的研究结果暗示了一种由转录因子精心策划的共同分层调节结构，用于专门的代谢和发育，使其成为代谢工程的一个有吸引力的目标。
The plant kingdom produces hundreds of thousands of specialized bioactive metabolites, some with pharmaceutical and biotechnological importance. Their biosynthesis and function have been studied for decades, but comparatively less is known about how transcription factors with overlapping functions and contrasting regulatory activities coordinately control the dynamics and output of plant specialized metabolism. Here, we performed temporal studies on pathogen-infected intact host plants with perturbed transcription factors. We identified WRKY33 as the condition-dependent master regulator and MYB51 as the dual functional regulator in a hierarchical gene network likely responsible for the gene expression dynamics and metabolic fluxes in the camalexin and 4-hydroxy-indole-3-carbonylnitrile (4OH-ICN) pathways. This network may have also facilitated the regulatory capture of the newly evolved 4OH-ICN pathway in Arabidopsis thaliana by the more-conserved transcription factor MYB51. It has long been held that the plasticity of plant specialized metabolism and the canalization of development should be differently regulated; our findings imply a common hierarchical regulatory architecture orchestrated by transcription factors for specialized metabolism and development, making it an attractive target for metabolic engineering.