The paraventricular nucleus of the thalamus as an interface between the orexin and CART peptides and the shell of the nucleus accumbens
The paraventricular nucleus of the thalamus as an interface between the orexin and CART peptides and the shell of the nucleus accumbens
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DOI：
10.1002/syn.20264
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发表时间：
2006-06-15
期刊：
影响因子：
2.3
通讯作者：
Kirouac, GJ
中科院分区：
文献类型：
--
作者：
Parsons, MP;Li, S;Kirouac, GJ
丘脑室旁核（PVT）接收来自脑干的传入，并被认为将唤醒相关信息传递到特定的边缘前脑区域，包括丘脑核。最近的解剖学观察表明，PVT也接受来自不同的下丘脑核团的传入。本解剖学实验研究了食欲素和可卡因和苯丙胺相关转录物（CART），两个高度集中在下丘脑的摄食相关肽的免疫反应纤维的PVT的神经支配。重点放在确定这些神经肽和PVT神经元投射到核壳（NacSh）之间的关系。逆行示踪剂注入NacSh标记的中线和intralaminar丘脑，其中大部分被限制在PVT的许多细胞。逆行示踪剂，食欲素纤维，CART纤维免疫阳性，而在整个PVT信号之间没有重叠是明显的相邻丘脑区域内。高倍率光学和共聚焦显微镜显示，orexin和CART纤维与整个前后PVT的逆行标记神经元频繁接触。此外，来自NacSh的逆行标记的单个PVT细胞与orexin和CART纤维并列。目前的实验提供了第一个证据，表明PVT作为下丘脑活动的中继到延髓核的作用。PVT的功能可能是将内脏唤醒信号与参与行为反应的边缘系统区域联系起来。
The paraventricular nucleus of the thalamus (PVT) receives afferents from the brainstem and has been thought to relay arousal related information to specific limbic forebrain areas, including the nucleus accumbens. More recent anatomical observations suggest that the PVT also receives afferents from various hypothalamic nuclei. The present anatomical experiments investigated the innervation of the PVT by fibers immunoreactive for orexin and cocaine and amphetamine related transcript (CART), two feeding-related peptides highly concentrated in the hypothalamus. Emphasis was placed on identifying the relationship between these neuropeptides and PVT neurons projecting to the shell of the nucleus accumbens (NacSh). Infusion of a retrograde tracer into the NacSh labeled numerous cells of the midline and intralaminar thalamus, most of which were restricted to the PVT. The retrograde tracer, orexin fibers, and CART fibers were immunopositive throughout the entire PVT whereas no overlap between signals was evident within adjacent thalamic regions. High-magnification light and confocal microscopy showed that both orexin and CART fibers made frequent contact with retrogradely labeled neurons throughout the anteroposterior PVT. Furthermore, single PVT cells retrogradely labeled from the NacSh were apposed by both orexin and CART fibers. The present experiments provide the first evidence suggesting a role for the PVT as a relay of hypothalamic activity to the nucleus accumbens. The PVT may function to link visceral arousal signals with limbic regions involved in behavioral responses.