Influence of low- and high-elevation plant genomes on the regulation of autumn cold acclimation in Abies sachalinensis.
Influence of low- and high-elevation plant genomes on the regulation of autumn cold acclimation in Abies sachalinensis.
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DOI：
10.3389/fpls.2015.00890
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发表时间：
2015
影响因子：
5.6
通讯作者：
Goto S
中科院分区：
文献类型：
--
作者：
Ishizuka W;Ono K;Hara T;Goto S
地理分布广泛的北方针叶树种在种群间秋季抗寒驯化方面存在显著差异。为了确定这种差异在代际间是如何遗传的，我们进行了子代测试，并研究了库页冷杉自由授粉的第二代（F2）子代抗寒锻炼的发展情况。F1亲本由于低海拔（L）和高海拔（H）种群间的正反交而具有不同的遗传背景：L×L、L×H、H×L和H×H。利用分子遗传标记对F2子代进行的父本分析表明，91.3%的父本位于F1种植地周围的林分中（即不在F1测试种群中）。其余的父本被归为L×L杂交类型的F1亲本。这表明F1亲本中的高海拔基因组没有通过花粉流遗传给F2种群。F2子代秋季抗寒驯化的时间取决于F1母本的杂交类型。在冷冻测试中，H×H母本的子代比其他杂交类型的子代受到的损伤更小。统计建模支持基因组来源的线性效应。在最佳模型中，冷冻损伤的差异由母本遗传的高海拔基因组的比例来解释。这些结果表明，秋季抗寒驯化部分由相关母本基因组的加性效应来解释。因此，遗传了更高比例高海拔基因组的后代更早地发展出抗寒性。秋季抗寒驯化调控中基于基因组的差异与当地气候条件相匹配，这可能是海拔相关适应的一个关键因素。
Boreal coniferous species with wide geographic distributions show substantial variation in autumn cold acclimation among populations. To determine how this variation is inherited across generations, we conducted a progeny test and examined the development of cold hardening in open-pollinated second-generation (F2) progeny of Abies sachalinensis. The F1 parents had different genetic backgrounds resulting from reciprocal interpopulational crosses between low-elevation (L) and high-elevation (H) populations: L × L, L × H, H × L, and H × H. Paternity analysis of the F2 progeny using molecular genetic markers showed that 91.3% of the fathers were located in surrounding stands of the F1 planting site (i.e., not in the F1 test population). The remaining fathers were assigned to F1 parents of the L × L cross-type. This indicates that the high-elevation genome in the F1 parents was not inherited by the F2 population via pollen flow. The timing of autumn cold acclimation in the F2 progeny depended on the cross-type of the F1 mother. The progeny of H × H mothers showed less damage in freezing tests than the progeny of other cross-types. Statistical modeling supported a linear effect of genome origin. In the best model, variation in freezing damage was explained by the proportion of maternally inherited high-elevation genome. These results suggest that autumn cold acclimation was partly explained by the additive effect of the responsible maternal genome. Thus, the offspring that inherited a greater proportion of the high-elevation genome developed cold hardiness earlier. Genome-based variation in the regulation of autumn cold acclimation matched the local climatic conditions, which may be a key factor in elevation-dependent adaptation.