Cellular aging dynamics after acute malaria infection: A 12-month longitudinal study
Cellular aging dynamics after acute malaria infection: A 12-month longitudinal study
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DOI：
10.1111/acel.12702
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发表时间：
2018-02-01
期刊：
影响因子：
7.8
通讯作者：
Farnert, Anna
中科院分区：
文献类型：
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作者：
Asghar, Muhammad;Yman, Victor;Farnert, Anna
最近，慢性感染疟原虫的鸟类的细胞老化速度加快，寿命缩短。疟疾感染是否也会影响人类细胞衰老尚未有报道。在这里，我们评估了一次急性恶性疟原虫疟疾感染对瑞典旅行者一年多的前瞻性随访中细胞衰老动态的影响。从入院时收集的静脉血中提取 DNA 和 RNA，并在一年内重复提取。使用实时定量 PCR 测量端粒长度，通过逆转录酶 (RT)-qPCR 测量端粒酶活性和 CDKN2A 表达。我们的结果表明，急性疟疾感染会影响细胞衰老，表现为感染后头三个月内 CDKN2A 表达水平升高、端粒酶活性降低以及端粒显着缩短。 CDKN2A表达下降后，端粒酶活性增加，端粒长度在一年内逐渐恢复，反映出细胞衰老被逆转。这些发现表明，疟疾感染会影响细胞衰老，并且需要阐明病原体影响宿主细胞衰老和寿命的潜在细胞机制。我们的研究结果表明，有必要调查疟疾反复感染是否对疟疾流行地区人群的细胞衰老和寿命（类似于在鸟类中观察到的情况）产生更明显和持久的影响。
Accelerated cellular aging and reduced lifespan have recently been shown in birds chronically infected with malaria parasites. Whether malaria infection also affects cellular aging in humans has not been reported. Here, we assessed the effect of a single acute Plasmodium falciparum malaria infection on cellular aging dynamics in travelers prospectively followed over one year in Sweden. DNA and RNA were extracted from venous blood collected at the time of admission and repeatedly up to one year. Telomere length was measured using real-time quantitative PCR, while telomerase activity and CDKN2A expression were measured by reverse transcriptase (RT)-qPCR. Our results show that acute malaria infection affects cellular aging as reflected by elevated levels of CDKN2A expression, lower telomerase activity, and substantial telomere shortening during the first three months postinfection. After that CDKN2A expression declined, telomerase activity increased and telomere length was gradually restored over one year, reflecting that cellular aging was reversed. These findings demonstrate that malaria infection affects cellular aging and the underlying cellular mechanism by which pathogens can affect host cellular aging and longevity need to be elucidated. Our results urge the need to investigate whether repeated malaria infections have more pronounced and long-lasting effects on cellular aging and lifespan (similarly to what was observed in birds) in populations living in malaria endemic areas.