High-throughput precision measurement of subcellular localization in single cells
High-throughput precision measurement of subcellular localization in single cells
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DOI：
10.1002/cyto.a.23054
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发表时间：
2017-01-01
期刊：
影响因子：
3.7
通讯作者：
Nolan, Garry P.
中科院分区：
文献类型：
--
作者：
Burns, Tyler J.;Frei, Andreas P.;Nolan, Garry P.
为了以每秒数千个细胞的吞吐量量化单个细胞中的视觉和空间信息，我们开发了亚细胞定位测定（SLA）。这种对邻近连接测定的修改扩展了流式细胞术的能力，以包括与蛋白质定位到细胞器和细胞器内相关的数据。我们使用SLA来检测复杂样品中跨细胞亚群的转录因子的核输入。我们进一步测量了在整个细胞周期和DNA损伤诱导后靶蛋白的核内重新定位。SLA结合了多种单细胞方法，为复杂细胞群的查询和分析带来了新的维度。(c)2017年国际细胞计数促进学会
To quantify visual and spatial information in single cells with a throughput of thousands of cells per second, we developed Subcellular Localization Assay (SLA). This adaptation of Proximity Ligation Assay expands the capabilities of flow cytometry to include data relating to localization of proteins to and within organelles. We used SLA to detect the nuclear import of transcription factors across cell subsets in complex samples. We further measured intranuclear re-localization of target proteins across the cell cycle and upon DNA damage induction. SLA combines multiple single-cell methods to bring about a new dimension of inquiry and analysis in complex cell populations. (c) 2017 International Society for Advancement of Cytometry