Sex differences in forkhead box O3a signaling response to hindlimb unloading in rat soleus muscle
Sex differences in forkhead box O3a signaling response to hindlimb unloading in rat soleus muscle
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DOI：
10.1007/s12576-018-0640-6
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发表时间：
2019-03-01
影响因子：
2.3
通讯作者：
Naito, Hisashi
中科院分区：
文献类型：
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作者：
Yoshihara, Toshinori;Natsume, Toshiharu;Naito, Hisashi
我们测试的假设，有性别差异的后肢卸载诱导激活的叉头盒亚家族O3 a（FoxO 3a）信号通路在大鼠比目鱼肌。雌雄配对的Wistar大鼠进行后肢去负荷实验，分别于去负荷前、去负荷后1天或7天切除比目鱼肌。雌性大鼠比目鱼肌相对重量的百分比变化大于雄性大鼠。FoxO 3a磷酸化在女性中低于男性，并与卸载后7天的蛋白质泛素化水平较高相关。热休克蛋白72（HSP 72）水平较低，雌性大鼠和增加在卸载过程中的男性。雌性大鼠显示肌肉生长抑制素水平略高，雄性大鼠在卸载后显示非显著性下降。因此，男性和女性表现出不同的反应FoxO 3a/泛素-蛋白酶体途径后，后肢卸载大鼠比目鱼肌，这可能与热休克蛋白72表达和肌肉生长抑制素信号的差异。
We tested the hypothesis that there are sex differences in hindlimb unloading-induced activation of the forkhead box subfamily O3a (FoxO3a) signaling pathway in rat soleus muscle. Age-matched male and female Wistar rats were subjected to hindlimb unloading, and the soleus muscle was removed before or 1 or 7days after unloading. Female rats showed greater percent changes in relative soleus muscle weight than males. FoxO3a phosphorylation was lower in females than in males and was associated with higher levels of protein ubiquitination 7days after unloading. Heat shock protein 72 (Hsp72) levels were lower in female rats and increased in males during unloading. Female rats showed slightly higher myostatin levels, which showed a non-significant decline in male rats following unloading. Thus, males and females show different responses to the FoxO3a/ubiquitin-proteasome pathway following hindlimb unloading in rat soleus muscle, which may be associated with differences in Hsp72 expression and myostatin signaling.