Attenuated satiation response to intestinal nutrients in rats that do not express CCK-A receptors
Attenuated satiation response to intestinal nutrients in rats that do not express CCK-A receptors
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DOI：
10.1016/s0196-9781(01)00461-2
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发表时间：
2001-08-01
期刊：
影响因子：
3
通讯作者：
Ritter, RC
中科院分区：
文献类型：
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作者：
Covasa, M;Ritter, RC
药理学实验表明，与肠道输注几种营养素有关的饱足感是由CCK-A受体介导的。大冢龙-埃文斯德岛肥胖大鼠（OLETF）不表达CCK-A受体，对外源性CCK产生饱腹感的作用不敏感。为了进一步评价CCK-A受体在肠道营养输注饱腹感中的作用，我们检查了OLETF大鼠和Long Evans德岛Orsuka对照大鼠（LETO）在肠道输注脂肪（油酸或脂肪乳剂）、糖（麦芽三糖或葡萄糖）或蛋白胨后的固体（颗粒状大鼠食物）或液体（12.5%葡萄糖）食物摄入。肠输注葡萄糖或麦芽三糖减少固体食物摄入量LETO比OLETF大鼠从30分钟到4小时后输注。在输注后的前30分钟内，通过肠内输注脂肪或蛋白胨减少固体食物摄入在OLETF和LETO大鼠之间没有差异，但在输注后随后的4小时内，这些输注物减少OLETF大鼠的摄入量。肠道输注葡萄糖，油酸，脂肪乳剂和蛋白胨减少30分钟摄入量的12.5%的葡萄糖LETO比OLETF大鼠。此外，CCK-A受体拮抗剂，devazepide预处理，衰减肠营养诱导的食物摄入量减少，只有在LETO，但坚果OLETF大鼠。我们的研究结果证实了药理学结果，表明CCK-A受体参与饱食的营养素，提高血浆CCK浓度，以及营养素，不刺激分泌的内分泌CCK。此外，我们的研究结果表明：1）OLETF大鼠对各种肠道营养素输注的饱足反应存在缺陷; 2）在摄入液体和固体食物期间，CCK-A受体参与肠道营养素饱足的时间模式不同; 3）肠道营养素提供一些由CCK-A受体介导的饱足信号，而一些则不是。(C)2001 Elsevier Science Inc. All rights reserved.
Pharmacological experiments suggest that satiation associated with intestinal infusion of several nutrients is mediated by CCK-A receptors. Otsuka Long-Evans Tokushima Fatty, (OLETF), rats do not express CCK-A receptors and are insensitive to the satiation-producing effects of exogenous CCK. To further evaluate the role of CCK-A receptors in satiation by intestinal nutrient infusion, we examined intake of solid (pelleted rat chow) or liquid (12.5% glucose) food intake, following intestinal infusions of fats (oleic acid or fat emulsion), sugars (maltotriose or glucose), or peptone in OLETF rats and Long Evans Tokushima Orsuka control rats (LETO). Intestinal infusion of glucose or maltotriose reduced solid food intake more in LETO than in OLETF rats from 30 min through 4 h post infusion. Reduction of solid food intake by intestinal infusions of fat or peptone did not differ between OLETF and LETO rats during the first 30 min post infusion, but reduction of intake by these infusates was attenuated in OLETF rats over the ensuing 4h post infusion. Intestinal infusion of glucose, oleate, fat emulsion and peptone reduced 30-min intake of 12.5% glucose more in LETO than OLETF rats. Furthermore, pretreatment with the CCK-A receptor antagonist, devazepide, attenuated intestinal nutrient-induced reduction of food intake only in LETO, but nut OLETF rats. Our results confirm pharmacological results, indicating that CCK-A receptors participate in satiation by nutrients that elevate plasma CCK concentrations, as well as by nutrients that do not stimulate secretion of endocrine CCK. In addition, our results indicate: 1) that OLETF rats have deficits in the satiation response to a variety of intestinal nutrient infusions; 2) that the temporal pattern for CCK-A receptor participation in satiation by intestinal nutrients is different during ingestion of liquid and solid feuds and 3) that intestinal nutrients provide some satiation signals that are CCK-A receptor mediated and some that are not. (C) 2001 Elsevier Science Inc. All rights reserved.