Leaves and stolons transcriptomic analysis provide insight into the role of phytochrome F in potato flowering and tuberization.
Leaves and stolons transcriptomic analysis provide insight into the role of phytochrome F in potato flowering and tuberization.
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DOI：
10.1111/tpj.16056
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发表时间：
2022-12
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影响因子：
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通讯作者：
Enshuang Wang;Tingting Zhou;S. Jing;Liepeng Dong;Xiaomeng Sun;Yujie Fan;Yunlong Shen;Tengfei Liu;Botao Song
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文献类型：
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作者：
Enshuang Wang;Tingting Zhou;S. Jing;Liepeng Dong;Xiaomeng Sun;Yujie Fan;Yunlong Shen;Tengfei Liu;Botao Song
光周期对马铃薯有性生殖器官和营养生殖器官的形成起着重要的控制作用。虽然StPHYF沉默的植物克服了日长的限制，通过对匍匐茎中的tuberigen StSP 6A表达的系统性影响来块茎化，但StPHYF的全面调控网络仍然不清楚。因此，本研究调查了转录组的StPHYF沉默的植物，并观察到，除了已知的光周期块茎化途径的组件，成花素StSP 3D和其他开花相关的基因被激活的StPHYF沉默的植物，表现出早期开花反应。此外，嫁接实验揭示了StPHYF沉默对匍匐茎中基因表达的长距离影响，包括生物钟成分，开花相关的MADS和结瘤相关的调控基因。与拟南芥中的AtFT-AtAP 1调控模块类似，本研究确定了AP 1样StMADS 1在块茎原激活复合物（TAC）下游发挥作用，并且抑制StMADS 1抑制体外块茎形成并延迟体内块茎形成。此外，StSP 6A的表达下调，在StMADS 1沉默的植物，这意味着StSP 6A的表达可能是由StMADS 1反馈调节。总体而言，这些结果表明，调控网络的StPHYF控制开花和结薯，并通过TAC的关键结薯因子StMADS 1的目标，从而提供了一个更好的理解StPHYF介导的日长感知在马铃薯繁殖。
Photoperiod plays a critical role in controlling the formation of sexual or vegetative reproductive organs in potato. Although StPHYF-silenced plants overcome day-length limitations to tuberize through a systemic effect on tuberigen StSP6A expression in the stolon, the comprehensive regulatory network of StPHYF remains obscure. Therefore, the present study investigated the transcriptomes of StPHYF-silenced plants and observed that, in addition to known components of the photoperiodic tuberization pathway, florigen StSP3D and other flowering-related genes were activated in StPHYF-silenced plants, exhibiting an early flowering response. Additionally, grafting experiments uncovered the long-distance effect of StPHYF silencing on gene expression in the stolon, including the circadian clock components, flowering-associated MADSs, and tuberization-related regulatory genes. Similar to the AtFT-AtAP1 regulatory module in Arabidopsis, the present study established that the AP1-like StMADS1 functions downstream of the tuberigen activation complex (TAC) and that suppressing StMADS1 inhibits tuberization in vitro and delays tuberization in vivo. Moreover, the expression of StSP6A was downregulated in StMADS1-silenced plants, implying the expression of StSP6A may be feedback-regulated by StMADS1. Overall, these results reveal that the regulatory network of StPHYF controls flowering and tuberization and targets the crucial tuberization factor StMADS1 through TAC, thereby providing a better understanding of StPHYF-mediated day-length perception during potato reproduction.