ESSENTIAL ROLE OF THE HMG DOMAIN IN THE FUNCTION OF YEAST MITOCHONDRIAL HISTONE HM - FUNCTIONAL COMPLEMENTATION OF HM BY THE NUCLEAR NONHISTONE PROTEIN NHP6A
ESSENTIAL ROLE OF THE HMG DOMAIN IN THE FUNCTION OF YEAST MITOCHONDRIAL HISTONE HM - FUNCTIONAL COMPLEMENTATION OF HM BY THE NUCLEAR NONHISTONE PROTEIN NHP6A
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DOI：
10.1073/pnas.90.12.5598
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发表时间：
1993-06-15
影响因子：
11.1
通讯作者：
CHAE, CB
中科院分区：
文献类型：
--
作者：
KAO, LR;MEGRAW, TL;CHAE, CB
酵母线粒体组蛋白HM是维持线粒体基因组所必需的，并且编码HM的基因（HIM 1/ABF 2）的破坏导致形成呼吸缺陷型娇小突变表型。HM含有两个同源区域，其与真核细胞核非组蛋白HMG-1共享序列相似性。用HM的各种缺失突变体进行的实验表明HM的单个HMG结构域是功能性的，并且可以恢复缺乏HM蛋白的细胞（him 1突变细胞）的呼吸能力。编码推定的酵母核HMG-1同源物的基因，NHP 6A蛋白，可以在功能上补充him 1突变。这些结果表明，HMG结构域是HM在线粒体中功能的基本单位，并且HMG-1蛋白在细胞核中的功能和HM在线粒体中的功能可能是等同的。
The yeast mitochondrial histone protein HM is required for maintenance of the mitochondrial genome, and disruption of the gene encoding HM (HIM1/ABF2) results in formation of a respiration-deficient petite mutant phenotype. HM contains two homologous regions, which share sequence similarity with the eukaryotic nuclear nonhistone protein, HMG-1. Experiments with various deletion mutants of HM show that a single HMG domain of HM is functional and can restore respiration competency to cells that lack HM protein (him1 mutant cells). The gene encoding the putative yeast nuclear HMG-1 homolog, the NHP6A protein, can functionally complement the him1 mutation. These results suggest that the HMG domain is the basic unit for the function of HM in mitochondria and that the function of HMG-1 proteins in the nucleus and HM in the mitochondrion may be equivalent.