Contralateral neuropathic pain following a surgical model of unilateral nerve injury in rats
Contralateral neuropathic pain following a surgical model of unilateral nerve injury in rats
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DOI：
10.1016/j.rapm.2007.12.003
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发表时间：
2008-05-01
影响因子：
5.1
通讯作者：
Gomar, Carmen
中科院分区：
文献类型：
--
作者：
Arguis, Maria Jose;Perez, Jordi;Gomar, Carmen
背景和目的：周围神经损伤引起慢性神经病理性疼痛，持续时间超过神经损伤的持续时间。据报道，疼痛体征存在于除归因于受损神经的区域之外的区域，即，在对侧区域。我们评估的存在，幅度，和时序的机械和冷异常性疼痛在对侧爪的大鼠进行单侧结扎的L5和L 6脊nerves.Methods：23只雄性Sprague-Dawley大鼠进行脊神经结扎的左侧L5和L 6脊神经（SNL组）和7只大鼠接受了假手术没有神经结扎（假手术组）。在手术前和术后21天内，研究了用von Frey细丝评估的机械性异常性疼痛体征和用丙酮跌落试验评估的冷异常性疼痛体征。结果：SNL组大鼠同侧足爪出现机械性和冷触诱发痛反应，对侧足也出现机械性和冷触诱发痛反应。对侧爪的异常性疼痛出现较晚，与假手术组相比，机械性异常性疼痛在手术后第10天变得具有统计学显著性，冷性异常性疼痛在手术后第21天变得具有统计学显著性。同侧疼痛的强度低于issilateralsides.Conclusions：L5和L 6脊神经结扎后，大鼠开发的机械和冷异常性疼痛在对侧爪，表明域外发展的神经病变体征。这一发现对未来的研究、设计和治疗方法具有启示意义。
Background and Objectives: Damage to peripheral nerves provokes chronic neuropathic pain that lasts beyond the duration of the nerve injury. The presence of pain signs have been reported in areas other than those attributed to the injured nerve, i.e., in contralateral regions. We evaluated the presence, magnitude, and chronology of mechanical and cold allodynia in the contralateral paw of rats undergoing unilateral ligation of the L5 and L6 spinal nerves.Methods: Twenty-three male Sprague-Dawley rats underwent spinal nerve ligation of the left L5 and L6 spinal nerves (SNL group) and 7 rats received a sham surgery without nerve ligation (sham group). Signs of mechanical allodynia as assessed with von Frey filaments, and cold allodynia as assessed with the acetone drop test, were studied before surgery and throughout 21 postoperative days. Responses of ipsilateral and contralateral paws of the SNL group were compared between themselves and with those in the sham group.Results: Rats in the SNL group developed mechanical and cold allodynia responses in the ipsilateral paw, and also in the contralateral paw. Allodynia in the contralateral paw appeared later, becoming statistically significant on day 10 after surgery for mechanical allodynia and on day 21 for cold allodynia as compared with the sham group. Contralateral pain was of a lower intensity than on the ipsilateral side.Conclusions: After L5 and L6 spinal nerve ligation, rats developed mechanical and cold allodynia in the contralateral paw, suggesting extraterritorial development of neuropathic signs. This finding has implications for future study,design and therapeutic approaches.