Antidepressant-like effects of ginsenoside Rg2 in a chronic mild stress model of depression
Antidepressant-like effects of ginsenoside Rg2 in a chronic mild stress model of depression
复制标题
人参皂苷 Rg2 在抑郁症慢性轻度应激模型中的抗抑郁样作用
DOI：
10.1016/j.brainresbull.2017.08.009
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发表时间：
2017-09-01
影响因子：
3.8
通讯作者：
Jiang, Bo
中科院分区：
文献类型：
--
作者：
Ren, Ying;Wang, Jin-Liang;Jiang, Bo
严重抑郁症是一种常见的神经精神疾病，终生患病率很高，自杀发生率很高。这项研究旨在评估人参糖苷RG2在小鼠中的抗抑郁作用，并确定了可能的机制。在强制游泳测试和尾部悬架测试中，单次注射RG2（10和20 mg/kg）和氟西汀（阳性对照，20 mg/kg）诱导了明显的抗抑郁样作用，而不会影响小鼠的运动活性，以及注射后30分钟进行测试。同样，在过去的2周中，每天重复对RG2和氟西汀进行重复治疗，完全逆转了慢性轻度应激（6周）诱导小鼠抑郁样症状。此外，Western印迹分析表明，RG2给药可显着增加海马中的BDNF信号通路。重要的是，TRKB shRNA的使用完全阻断了RG2对小鼠的抗抑郁作用。总的来说，这些结果表明，RG2在小鼠中产生抗抑郁样作用，至少部分通过促进海马BDNF信号通路介导。
Major depression is a common neuropsychiatric disease with high lifetime prevalence and high incidence of suicide. This study aimed to evaluate the antidepressant effects of ginsenoside Rg2 in mice, and the possible mechanism was also determined. A single injection of both Rg2 (10 and 20 mg/kg) and fluoxetine (positive control, 20 mg/kg) induced notable antidepressant-like effects in the forced swim test and tail suspension test without affecting the locomotor activity of mice, and the tests were done 30min after the injection. Also, repeated daily treatment of Rg2 and fluoxetine for the last 2 weeks fully reversed the chronic mild stress (6 weeks) induced depressive-like symptoms in mice. Moreover, western blot analysis showed that Rg2 administration significantly increased the BDNF signaling pathway in hippocampus. Importantly, the usage of TrkB shRNA fully blocked the antidepressant effects of Rg2 in mice. Collectively, these results suggest that Rg2 produces an antidepressant-like effect in mice which is mediated, at least in part, through promoting the hippocampal BDNF signaling pathway.