Mesopore‐Induced Aggregation of Cobalt Protoporphyrin for Photoacoustic Imaging and Antioxidant Protection of Stem Cells
Mesopore‐Induced Aggregation of Cobalt Protoporphyrin for Photoacoustic Imaging and Antioxidant Protection of Stem Cells
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DOI：
10.1002/adfm.201804497
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发表时间：
2018-10
影响因子：
19
通讯作者：
Minghua Yao;Ming Ma;Hongbo Zhang;Yuezhou Zhang;Gang Wan;Jie Shen;Hangrong Chen;Rong Wu
中科院分区：
文献类型：
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作者：
Minghua Yao;Ming Ma;Hongbo Zhang;Yuezhou Zhang;Gang Wan;Jie Shen;Hangrong Chen;Rong Wu
随着功能材料设计和制造的不断加速发展，各种基于纳米材料的分子成像平台具有更好的可检测性和功能性，已被开发用于干细胞治疗领域的下一代细胞跟踪技术。在这项工作中，开发了一种“介孔诱导聚集”策略，通过将钴原卟啉IX（CoPP）负载到胺官能化的介孔二氧化硅纳米颗粒（aMSNs）中，赋予其光声（PA）成像能力。通过在介孔中的分子间聚集，CoPP的光吸收可以扩展到近红外区域，导致与游离CoPP相比，在680 nm处的PA响应增强约7.4倍。此外，新设计的PA造影剂不仅对干细胞追踪安全，而且还可持续释放CoPP，对氧化应激产生长期细胞保护作用。基于皮下和心肌注射方法，通过在裸鼠中对标记的干细胞进行体内成像，证明了负载CoPP的MSN作为治疗诊断剂的转化潜力。这是第一次展示具有PA成像引导细胞递送和抗氧化应激细胞保护功能的多功能纳米平台，为未来的干细胞治疗提供了强大的治疗诊断方法。
With the ever‐accelerating development of functional materials design and fabrication, various nanomaterial‐based molecular imaging platforms with improved detectability and functionality have been developed for next‐generation cell tracking technologies in the field of stem cell therapy. In this work, a “mesopore‐induced aggregation” strategy is developed to endow cobalt protoporphyrin IX (CoPP), one of the important metalloporphyrins, with photoacoustic (PA) imaging capability by loading it into amine‐functionalized mesoporous silica nanoparticles (aMSNs). Through intermolecular aggregation in mesopores, the light absorption of CoPP can be broaden into near‐infrared region, resulting in about 7.4‐fold enhancement of PA response at 680 nm compared to free CoPP. Moreover, the newly designed PA contrast agent is not only safe for stem cell tracking, but also exerts sustained CoPP release for a long‐term cytoprotective effect against oxidative stress. The translational potential of CoPP‐loaded MSNs as a theranostic agent is demonstrated via in vivo imaging of the labeled stem cell in nude mice based on subcutaneous‐ and myocardial‐injection approaches. This is the first demonstration of a multifunctional nanoplatform with both PA imaging‐guided cell delivery and antioxidant stress cell protection functions, which provides a robust theranostic approach for future stem cell therapy.