A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.
A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.
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大肠杆菌的一种静止期蛋白，影响 trp 阻遏蛋白与操纵基因 DNA 之间的关联模式。
DOI：
10.1073/pnas.90.12.5796
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发表时间：
1993
影响因子：
11.1
通讯作者：
Somerville,RL
中科院分区：
文献类型：
--
作者：
Yang,W;Ni,L;Somerville,RL
发现来自大肠杆菌菌株的trp阻遏物（TrpR）蛋白的高度纯化的制剂含有另一种21 kDa的蛋白，所述大肠杆菌菌株被工程化以过表达该物质。第二种蛋白质，命名为Tryptophan（W）阻遏物结合蛋白，通过几个连续的蛋白质分级分离步骤与其同名蛋白保持相关。wrbA蛋白的N-末端氨基酸序列指导了两个简并寡核苷酸的设计，这两个简并寡核苷酸在wrbA基因（198个密码子）的克隆中用作探针。通过几个标准发现，纯化形式的TribA蛋白能够增强TrpR全抑制子与其主要操纵子靶标之间非共价复合物的形成和/或稳定性。凝胶迁移率-位移分析表明形成了一个操纵子-holorepressor-BABA三元复合物。单独的TribA蛋白不与trp操纵子相互作用。在稳定期，细胞中缺乏的蛋白质是有效的抑制trp启动子的能力比野生型。有人提出，在阻断TrpR特异性的转录过程，这可能是生理上不利的细菌生命周期的稳定期中的辅助元件的BABA蛋白的功能。
Highly purified preparations of trp repressor (TrpR) protein derived from Escherichia coli strains that were engineered to overexpress this material were found to contain another protein, of 21 kDa. The second protein, designated WrbA [for tryptophan (W) repressor-binding protein] remained associated with its namesake through several sequential protein fractionation steps. The N-terminal amino acid sequence of the WrbA protein guided the design of two degenerate oligonucleotides that were used as probes in the cloning of the wrbA gene (198 codons). The WrbA protein, in purified form, was found by several criteria to enhance the formation and/or stability of noncovalent complexes between TrpR holorepressor and its primary operator targets. The formation of an operator-holorepressor-WrbA ternary complex was demonstrated by gel mobility-shift analysis. The WrbA protein alone does not interact with the trp operator. During the stationary phase, cells deficient in the WrbA protein were less efficient than wild type in their ability to repress the trp promoter. It is proposed that the WrbA protein functions as an accessory element in blocking TrpR-specific transcriptional processes that might be physiologically disadvantageous in the stationary phase of the bacterial life cycle.
“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
Julen Castillo;Unai Arzubiaga;Jesús Manuel Palma
通讯作者：
Jesús Manuel Palma