Shedding of foodborne pathogens by Caenorhabditis elegans in compost-amended and unamended soil
Shedding of foodborne pathogens by Caenorhabditis elegans in compost-amended and unamended soil
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DOI：
10.1016/j.fm.2005.01.018
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发表时间：
2006-04-01
影响因子：
5.3
通讯作者：
Williams, PL
中科院分区：
文献类型：
--
作者：
Anderson, GL;Kenney, SJ;Williams, PL
进行了一项研究，以描述秀丽隐杆线虫对食源性致病菌的脱落，评估与食源性致病菌共培养的蠕虫种群的持久性，并确定秀丽隐杆线虫是否会因脱落而将摄入的病原体分散在土壤中。埃希氏菌大肠杆菌O157：H7、肠道沙门氏菌血清型Poona、单核细胞增生李斯特菌以及非致病性大肠杆菌OP 50。线虫的同步种群在萘啶酸适应细菌的汇合草坪上饲养24小时。线虫在喂食后5小时内，在添加萘啶酸（50 μ g ml（-1））（TSAN）的胰蛋白酶大豆琼脂上脱落摄入细菌的活细胞。秀丽线虫以在TSAN上脱落和生长的细菌为食，可以持续长达10天。从在棚食源性病原体上培养的秀丽隐杆线虫收获的卵具有与从在棚E上生长的秀丽隐杆线虫收集的卵相同的存活力水平。coli OP 50。在6-7天后，分别有78%、64%、64%和76%的线虫产卵。coliO157：H7、S.普纳湖inonocytogenes和E. coliOP50中表达。以E.大肠杆菌O15TH7接种到土壤和用火鸡粪便堆肥改良的土壤中。线虫种群在堆肥改良土壤中持续了至少7天，但在未改良土壤中有所下降。e.大肠杆菌O157：H7在接种后第4天和第6天在堆肥改良和未改良的土壤中均能检出，而在未改良的土壤中接种E. coli OP 50。种群E.大肠杆菌O157：H7在施用火鸡粪便堆肥的土壤中显著高于未施用堆肥的土壤（α = 0.05）。结果表明，线虫可以作为一种载体，分散土壤中的食源性病原体，可能导致收获前水果和蔬菜表面污染的风险增加。(c)2005爱思唯尔有限公司保留所有权利。
A study was done to characterize the shedding of foodborne pathogenic bacteria by Caenorhabditis elegans, evaluate the persistence of worm populations cocultured with foodborne pathogens, and determine if C elegans disperses ingested pathogens in soil as a result of shedding. Escherichia. coli O157:H7, Salmonella enterica serotype Poona, and Listeria monocytogenes, as well as E coli OP50, a non-pathogenic strain, were studied. Synchronous populations of C elegans were fed for 24h on confluent lawns of nalidixic acid-adapted bacteria. C elegans shed viable cells of ingested bacteria on tryptic soy agar supplemented with nalidixic acid (50 mu g ml(-1)) (TSAN) throughout a 5-h post-feeding period. C elegans persisted for up to 10 days by feeding on bacteria that had been shed and grew on TSAN. Eggs harvested from C elegans cultured on shed foodborne pathogens had the same level of viability as those collected from C elegans grown on shed E. coli OP50. After 6-7 days, 78%, 64%, 64%, and 76% of eggs laid by C elegans that had fed on E. coli O157:H7, S. Poona, L. inonocytogenes, and E. coli OP50, respectively, were viable. Worms fed on E. coli O15TH7 were inoculated into soil and soil amended with turkey manure compost. Populations of C elegans persisted in compostamended soil for at least 7 days but declined in unamended soil. E. coli O157:H7 was detected at 4 and 6 days post inoculation in compost-amended and unamended soil, and in unamended soil inoculated with E. coli OP50. Populations of E. coli O157:H7 in soil amended with turkey manure compost were significantly(alpha = 0.05) higher than those in unamended soil. Results indicate that C elegans can act as a vector to disperse foodborne pathogens in soil, potentially resulting in increased risk of contaminating the surface of pre-harvest fruits and vegetables. (c) 2005 Elsevier Ltd. All rights reserved.