Effects of MK-801 and ethanol combinations on memory consolidation in CD1 mice: Involvement of GABAergic mechanisms
Effects of MK-801 and ethanol combinations on memory consolidation in CD1 mice: Involvement of GABAergic mechanisms
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DOI：
10.1006/nlme.2001.4029
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发表时间：
2002-05-01
影响因子：
2.7
通讯作者：
Castellano, C
中科院分区：
文献类型：
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作者：
Aversano, M;Ciamei, A;Castellano, C
在本研究中，非竞争性N-甲基-D-天冬氨酸受体拮抗剂MK-801和乙醇的组合对记忆巩固的效果和GABA能机制在这种效果中的参与进行了研究，在CD I小鼠在一次试验的抑制性回避装置中训练后立即或120分钟腹腔注射药物，并在24小时后测试保留。结果表明：（1）MK-801对小鼠记忆成绩有明显的影响，且呈剂量依赖性（0.2和0.3，但不是0.1 mg/kg）和乙醇（1和2，但不是0.5 g/kg）给药;（B）其它无效剂量的MK-801（0.1 mg/kg）可增强乙醇的毒性作用（1和2 g/kg）;（c）否则无效剂量的蝇蕈醇（0.5 mg/kg）增强，而其他无效剂量的印防己毒素（0.25 mg/kg）或荷包牡丹碱（0.1mg/kg）可拮抗此作用;和（d）当训练后120分钟进行处理时没有观察到效果，这表明训练后立即给药后观察到的效果是由于对记忆巩固的影响。从这些实验中可以明显看出，（a）MK-801增强了乙醇对记忆巩固的作用，（B）GABA能机制参与了该作用。(C)2001 Elsevier Science（美国）。
In the present research the effect of the noncompetitive N-methyl-D-aspartate receptor antagonist MK-801 and ethanol combinations on memory consolidation and the involvement of GABAergic mechanisms in this effect were investigated in CD I mice injected intraperitoneally with the drugs immediately or 120 min after training in a one-trial inhibitory avoidance apparatus and tested for retention 24 h later. The results showed that (a) the retention performances of mice were impaired in a dose-dependent manner by immediate posttraining MK-801 (0.2 and 0.3, but not 0.1 mg/kg) and ethanol (1 and 2, but not 0.5 g/kg) administrations; (b) an otherwise ineffective dose of MK-801 (0.1 mg/kg) enhanced the deleterious effect exerted by ethanol (1 and 2 g/kg); (c) an otherwise ineffective dose of muscimol (0.5 mg/kg) enhanced, while otherwise ineffective doses of picrotoxin (0.25 mg/kg) or bicuculline (0.1 mg/kg) antagonized, this effect; and (d) no effect was observed when the treatments were carried out 120 min after training, suggesting that the effects observed following immediate posttraining administrations were due to the influence on the consolidation of memory. From these experiments it is evident that (a) MK-801 enhances ethanol's effects on memory consolidation and (b) GABAergic mechanisms are involved in this effect. (C) 2001 Elsevier Science (USA).