Adhesion of selected Bifidobacterium strains to human intestinal mucus and the role of adhesion in enteropathogen exclusion
Adhesion of selected Bifidobacterium strains to human intestinal mucus and the role of adhesion in enteropathogen exclusion
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DOI：
10.4315/0362-028x-68.12.2672
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发表时间：
2005-12-01
影响因子：
2
通讯作者：
Salminen, S
中科院分区：
文献类型：
--
作者：
Collado, MC;Gueimonde, M;Salminen, S
潜在的益生菌菌株粘附于肠粘膜并排除和置换病原体的能力对于肠道微生物群的治疗操作是至关重要的。对7株人双歧杆菌和5株人肠道致病菌的粘附能力进行了分析，并与4株鸡肠道分离菌进行了比较。根据菌株的不同，双歧杆菌菌株的粘附范围为3 - 16%。双歧杆菌菌株的动物源性粘附显着优于菌株的人源性。在致病菌中，大肠杆菌NCTC 8603具有最高粘附值（20%），鼠伤寒沙门氏菌ATCC 29631、坂崎肠杆菌ATCC 29544和艰难梭菌ATCC 9689具有10 - 15%的粘附值，单核细胞增生李斯特菌ATCC 15313具有最低粘附值（3%）。还测试了这些双歧杆菌抑制病原体粘附和置换先前粘附于粘液的病原体的能力。这些双歧杆菌菌株对病原体粘附的抑制是可变的，并且明显依赖于菌株。一般来说，动物源性双歧杆菌菌株比人类菌株更能抑制和取代病原体。细菌粘附的初步表征完成使用不同的预处理，以探索粘附机制。结果表明，不同的分子参与了双歧杆菌对人类肠道粘液的粘附，构成了一个多因素的过程。
The ability of potential probiotic strains to adhere to the intestinal mucosa and exclude and displace pathogens is of utmost importance for therapeutic manipulation of the enteric microbiota. The ability of seven selected human bifidobacterial strains and five human enteropathogenic strains to adhere to human intestinal mucus was analyzed and compared with that of four strains isolated from chicken intestines. The adhesion of the bifidobacterial strains ranged from 3 to 16% depending on the strain. Bifidobacterium strains of animal origin adhered significantly better than did strains of human origin. Of the pathogenic bacteria, Escherichia coli NCTC 8603 had the highest adhesion value (20%), Salmonella Typhimurium ATCC 29631, Enterobacter sakazakii ATCC 29544, and Clostridium difficile ATCC 9689 had adhesion values ranging from 10 to 15%, and Listeria monocytogenes ATCC 15313 had the lowest adhesive value (3%). The ability of these bifidobacteria to inhibit pathogen adhesion and to displace pathogens previously adhering to mucus was also tested. The inhibition of pathogens adhesion by these bifidobacterial strains was variable and clearly strain dependent. In general, bifidobacterial strains of animal origin were better able to inhibit and displace pathogens than were human strains. Preliminary characterization of bacterial adhesion was accomplished using different pretreatments to explore adhesion mechanisms. The results indicate that different molecules are implicated in the adhesion of bifidobacteria to the human intestinal mucus, constituting a multifactorial process.