High resolution fundus imaging by confocal scanning laser ophthalmoscopy in the mouse
High resolution fundus imaging by confocal scanning laser ophthalmoscopy in the mouse
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DOI：
10.1016/j.visres.2005.09.037
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发表时间：
2006-04-01
期刊：
影响因子：
1.8
通讯作者：
Sahel, JA
中科院分区：
文献类型：
--
作者：
Paques, M;Sirnonutti, M;Sahel, JA
我们使用改良的共焦扫描激光检眼镜（cSLO）在小鼠中评估眼底成像。在清醒的未经训练的小鼠中进行检查。最大的视野测量1520 × 1520亩，具有显着的个体间变异性，本身与生物特征的变异性。复合视野一直延伸到锯齿缘。反射成像与神经纤维束和血管壁的光反射以及血红蛋白和黑色素的吸收有关。色素上皮的光吸收确实增加了神经纤维层的对比度，但损害了脉络膜的观察。由于共焦模式，即使在白化病小鼠中也可以获得视网膜和脉络膜荧光清晰分离的荧光血管造影。显微尺度的横向分辨率和几个平面内的视网膜光学切片。例如，这允许在CX（3）CR1小鼠中对表达GFP的视网膜小胶质细胞进行三维亚细胞观察。我们的结论是，cSLO是一种有前途的工具，非侵入性，多模式活体显微镜的眼底在小鼠。(c)2005爱思唯尔有限公司保留所有权利。
We evaluated fundus imaging using a modified confocal scanning laser ophthalmoscope (cSLO) in mice. Examinations were performed in conscious, untrained mice. The largest field of view measured 1520 x 1520 mu, with a significant interindividual variability, itself correlated to biometric variability. The composite field of view extended up to the ora serrata. The reflectance imaging associated light reflection from nerve fiber bundles and vessel walls, and absorption by hemoglobin and melanin. Light absorption by the pigment epithelium indeed increased the contrast of the nerve fiber layer, but impaired viewing of the choroid. Due to the confocal mode, fluorescence angiograms with clear separation of retinal and choroidal fluorescence could be obtained even in albino mice. Micrometric-scale transverse resolution and several planes of optical sectioning within the retina were obtained. This permitted for instance tridimensional, subcellular viewing of gfp-expressing retinal microglial cells in CX(3)CR1 mice. We concluded that cSLO is a promising tool for noninvasive, multimodal intravital microscopy of the fundus in the Mouse. (c) 2005 Elsevier Ltd. All rights reserved.