The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA.
The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA.
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RCC1蛋白是染色体凝结发作的调节剂，位于细胞核中并与DNA结合。
DOI：
10.1083/jcb.109.4.1389
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发表时间：
1989-10
影响因子：
7.8
通讯作者：
Nishimoto, T
中科院分区：
文献类型：
--
作者：
Ohtsubo, M;Okazaki, H;Nishimoto, T
发现RCC1基因是一种调节染色体冷凝的调节剂，它使用针对根据假定RCC1蛋白的氨基酸序列制备的抗体来编码分子质量为45 kD的蛋白质。位于核中的p45是通过DNase I消化或用0.3 m NaCl处理的分离核从分离的核中释放的。一致地，用0.3 m NaCl洗脱与DNA-纤维素柱结合的P45。在用DNase I和2 M NaCl进行顺序处理后，几乎所有RCC1蛋白都从细胞核中释放出来。因此，RCC1蛋白位于染色质上，不是核基质的组成部分。在有丝分裂细胞中，p45分散到细胞质中。据推测，RCC1蛋白在调节染色体凝结，转录水平或mRNA成熟水平上起作用。
The RCC1 gene, a regulator for the onset of chromosome condensation was found to encode a protein with a molecular mass of 45 kD, determined using the antibody against the synthetic peptides prepared according to the amino acid sequence of the putative RCC1 protein. The p45 located in the nuclei was released from the isolated nuclei, either by DNase I digestion or by treatment with 0.3 M NaCl. Consistently, p45 bound to the DNA-cellulose column was eluted with 0.3 M NaCl. After sequential treatment with DNase I and 2 M NaCl, almost all of the RCC1 protein were released from the nuclei. Thus, RCC1 protein locates on the chromatin and is not a component of the nuclear matrix. In mitotic cells, p45 is dispersed into the cytoplasm. Presumably, RCC1 protein plays a role in regulating the onset of chromosome condensation, at the level of transcription or of mRNA maturation.